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Invitrogen
CD33 Monoclonal Antibody (WM-53 (WM53)), eBioscience™
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FIGURE: 1 / 14
CD33 Antibody (14-0338-82) in Flow
Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human CD33 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the monocyte (left) and granulocyte (right) gates were used for analysis.
Product Details
14-0338-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
WM-53 (WM53)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.
Applications Reported: The WM-53 (WM53) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunocytochemistry staining.
Applications Tested: The WM-53 (WM53) antibody has been tested by flow cytometric analysis of lysed whole blood. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD33; CD33 antigen (gp67); CD33 molecule transcript; FLJ00391; gp67; lacks exon 2 but includes exon 7a; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; unnamed protein product
Gene Aliases: CD33; CD33rSiglec; p67; SIGLEC-3; SIGLEC3
UniProt ID: (Human) P20138
Entrez Gene ID: (Human) 945
immune response-inhibiting signal transduction
cell adhesion
signal transduction
cell-cell signaling
negative regulation of cell proliferation
negative regulation of interleukin-1 beta production
negative regulation of interleukin-8 production
negative regulation of tumor necrosis factor production
Fc-gamma receptor signaling pathway
positive regulation of protein secretion
cell-cell adhesion
negative regulation of monocyte activation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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