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Invitrogen
CD69 Monoclonal Antibody (H1.2F3), eBioscience™
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Product Details
14-0691-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
H1.2F3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.
Applications Reported: The H1.2F3 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. H1.2F3 has also been reported in in vitro cell activation. (Please use Functional Grade purified H1.2F3, Product # 16-0691, in functional assays).
Applications Tested: The H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD69; Early activation antigen CD69; Very Early Activation Antigen
Gene Aliases: 5830438K24Rik; AI452015; AIM; Cd69; VEA
UniProt ID: (Mouse) P37217
Entrez Gene ID: (Mouse) 12515
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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