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Invitrogen
Rat IgM Monoclonal Antibody (HIS40), eBioscience™
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FIGURE: 1 / 2
Rat IgM Antibody (14-0990-82) in Flow
Rat splenocytes were stained with 0.25 µg of Mouse IgG1 kappa Isotype Control (Product # 14-4714-85) (blue histogram) or 0.25 µg of IgM Monoclonal Antibody (purple histogram) followed by IgG Polyclonal Antibody, PE (Product # 12-4010-87). Total viable cells as determined by 7AAD (Product # 00-6993-50) staining were used for analysis.
Product Details
14-0990-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
HIS40
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The HIS40 monoclonal antibody reacts with the µ heavy chain of rat IgM. It does not react with other classes of rat immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated HIS40 is used as a detection secondary for rat IgM primary antibodies.
Applications Reported: The HIS40 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.
Applications Tested: The HIS40 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Rat IgM is an immunoglobulin M isotype antibody produced by B cells in rats. It plays a crucial role in the immune system by recognizing and neutralizing antigens, such as bacteria and viruses. Rat IgM has been extensively studied and is widely used in research applications, including immunology, microbiology, and biotechnology. It is commonly used as a control in experiments, as well as a diagnostic tool for detecting infectious diseases and autoimmune disorders. Researchers have developed various techniques for producing and purifying Rat IgM, including hybridoma technology, protein A/G chromatography, and affinity chromatography. Rat IgM is a valuable tool in the field of immunology, and its use has contributed to a better understanding of the immune system and the development of new treatments for various diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene Aliases: IgM; RGD1359202
Entrez Gene ID: (Rat) 299357
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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