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CD182 (CXCR2) Monoclonal Antibody (eBio5E8-C7-F10 (5E8-C7-F10)), eBioscience™
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FIGURE: 1 / 3
CD182 (CXCR2) Antibody (14-1829-82) in Flow
Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (left) or 0.25 µg of Anti-Human CD182 (CXCR2) Purified (right) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Cells in the large scatter population were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-1829-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
eBio5E8-C7-F10 (5E8-C7-F10)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBio5E8-C7-F10 monoclonal antibody reacts with human CD182 (CXCR2, IL-8Rbeta). CD182 is a 67-70 kDa member of the seven-transmembrane spanning G-protein coupled receptor (GPCR) family. CD182 is expressed as a homodimer, or a heterodimer with CD181 (CXCR1, IL-8Ralpha) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, -beta and -gamma, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.
Applications Reported: This eBio5E8-C7-F10 (5E8-C7-F10) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio5E8-C7-F10 (5E8-C7-F10) antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CXCR2 is a receptor for interleukin-8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to GRO/MGSA and NAP-2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; CD182 antigen; chemokine (CXC) receptor 2; CXC; CXC motif chemokine; CXC-R2; CXCR; CXCR-2; CXCR2 gene for IL8 receptor type B; GRO/MGSA receptor; high affinity interleukin-8 receptor B; Il-8 receptor b; IL-8 receptor beta; IL-8 receptor type 2; IL-8R B; interleukin 8 receptor type 2; interleukin 8 receptor, beta; interleukin-8 receptor type B; unnamed protein product
Gene Aliases: CD182; CDw128b; CMKAR2; IL8R2; IL8RA; IL8RB; SCN13; WHIMS2
UniProt ID: (Human) P25025
Entrez Gene ID: (Human) 3579
dendritic cell chemotaxis
chemotaxis
inflammatory response
immune response
cellular defense response
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
positive regulation of cell proliferation
calcium-mediated signaling
neutrophil chemotaxis
receptor internalization
interleukin-8-mediated signaling pathway
neutrophil activation
regulation of chemotaxis
chemokine-mediated signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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