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Invitrogen

CD4 Monoclonal Antibody (N1UG0), eBioscience™

6 Published Figures
9 References
View all (495) CD4 antibodies

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Product Details

14-2444-82

Applications
Tested Dilution
Publications

Western Blot (WB)

5 µg/mL
-

Immunohistochemistry (IHC)

-
View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

2.5-5 µg/mL
-

Immunocytochemistry (ICC/IF)

-
View 3 publications 3 publications
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

N1UG0

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572868

Product Specific Information

Description: The N1UG0 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The N1UG0 antibody is recommended for use in staining human formalin-fixed paraffin embedded tissue sections.

Applications Reported: This N1UG0 antibody has been reported for use in western blotting and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This N1UG0 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval at less than or equal to 2.5 µg/mL. This N1UG0 antibody has been tested by western blot on reduced PBMC and can be used at less than or equal to 5.0 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T4 surface glycoprotein precursor; unnamed protein product

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Gene Aliases: CD4; CD4mut; IMD79; Leu-3; OKT4D; T4

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)
virus receptor activity transmembrane signaling receptor activity receptor binding extracellular matrix structural constituent protein binding zinc ion binding lipid binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein complex binding signaling receptor activity interleukin-16 binding interleukin-16 receptor activity MHC class II protein binding identical protein binding protein homodimerization activity protein tyrosine kinase binding
Process(es)
positive regulation of protein phosphorylation adaptive immune response immune system process positive regulation of immune system process immune response cell adhesion signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol calcium-mediated signaling T cell differentiation macrophage differentiation response to estradiol maintenance of protein location in cell positive regulation of interleukin-2 production response to vitamin D positive regulation of kinase activity helper T cell enhancement of adaptive immune response interleukin-15-mediated signaling pathway positive regulation of T cell proliferation T cell activation positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade T cell selection response to ethanol positive regulation of monocyte differentiation positive regulation of protein kinase activity positive regulation of transcription, DNA-templated positive regulation of viral entry into host cell viral entry into host cell defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation positive regulation of multicellular organismal process regulation of calcium ion transport positive regulation of ERK1 and ERK2 cascade cellular response to granulocyte macrophage colony-stimulating factor stimulus response to methamphetamine hydrochloride cellular response to ionomycin
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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