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          • Primary Antibodies ›
          • Neuropilin 1 (CD304) Antibodies

          Invitrogen

          CD304 (Neuropilin-1) Monoclonal Antibody (XML1Y1), eBioscience™

          Advanced Verification
          View all (47) Neuropilin 1 (CD304) antibodies

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          Cite CD304 (Neuropilin-1) Monoclonal Antibody (XML1Y1), eBioscience™

          Additional Information:
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          • Antibody Testing Data (3)
          • Advanced Verification (2)
          CD304 (Neuropilin-1) Antibody in Immunocytochemistry (ICC/IF)
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          CD304 (Neuropilin-1) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          CD304 (Neuropilin-1) Antibody (14-3042-82) in ICC/IF

          Immunofluorescence analysis of CD304 (Neuropilin-1) was performed using MDA-MB-231 and SK-BR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD304 (Neuropilin-1) Monoclonal Antibody (XML1Y1), eBioscience™ (Product # 14-3042-82) at 5 microgram/mL con... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD304 (Neuropilin-1) Antibody in Immunocytochemistry (ICC/IF)
          CD304 (Neuropilin-1) Antibody in Immunocytochemistry (ICC/IF)
          CD304 (Neuropilin-1) Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD304 (Neuropilin-1) Antibody
          CD304 (Neuropilin-1) Antibody

          Product Details

          14-3042-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (Frozen) (IHC (F))

          1 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          1 - 5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          XML1Y1

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2572873

          Product Specific Information

          Description: The monoclonal antibody XML1Y1 recognizes CD304 (Neuropilin-1), a type 1 transmembrane glycoprotein present on the surface of multiple cell types, including: regulatory T cells (Tregs), NKT cells, DC, certain types of stem cells, neurons, endothelial cells, and some neoplastic cells. Neuropilin-1, in complex with plexins, serves as a co-receptor for type 3 semaphorins in growing neurons. It is also involved in the process of angiogenesis being a part of a functional receptor for VEGF in endothelial cells. In mouse Neuropilin-1 is expressed on thymus-derived natural Tregs but not peripherally induced Treg cells. In addition, transient expression on recently activated non-regulatory T cells has been observed. Neuropilin-1 has been used as a marker of recent thymic emigrants in the mouse iNKT cell population. It has been shown that Neuropilin-1 forms a complex with TGF beta receptors, activating TGF beta latent form (LAP-TGF beta 1) and augmenting canonic Smad2/3 signaling.

          This XML1Y1 antibody recognizes neuropilin-1 isoforms 1 and 2.

          Applications Reported: This XML1Y1 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, and immunocytochemistry.

          Applications Tested: This XML1Y1 antibody has been tested by immunohistochemistry of acetone-fixed frozen tissue sections and can be used at less than or equal to 1 µg/mL. This XML1Y1 antibody has been tested by immunocytochemistry of methanol-fixed bEND3 cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          Neuropilin 1 is a 923 amino acid containing membrane bound coreceptor to a tyrosine kinase receptor belonging to the neuropilin family with two CUB domains, two F5/8 type C domains and a MAM domain. Neuropilin 1 forms a heterodimer with NRP2 (Neuropilin 2) and is known to bind to plexin (PLXNB1) to form semaphorin receptors. Expressed mostly in the nervous system this receptor is implicated in a wide range of functions including in the development of the cardiovascular system, angiogenesis, repulsive axon guidance, formation of certain neuronal circuits, cell survival, control of neuronal migration and organogenesis outside the nervous system. This semaphorin receptor functions as receptors for both the semaphorins and vascular endothelial growth factor and is also a regulator of VEGF-induced angiogenesis. Neuropilin 1 may be involved in aggressive advanced prostate carcinoma and in the progression of breast cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Bioinformatics

          Protein Aliases: A5 protein; alternatively spliced; lacks exon 16; CD304; Neuropilin; Neuropilin-1; Neuropilin-1 precursor (A5 protein); Neuropilin1; NRP 1; receptor for VEGF165 and semaphorins class 3; sNRP 1; sNRP1; soluble NRP 1; soluble NRP1; transmembrane receptor; unnamed protein product; Vascular endothelial cell growth factor 165 receptor; VEGF 165 receptor

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          Gene Aliases: BDCA4; C530029I03; CD304; NP-1; NP1; NPN-1; Npn1; NRP; NRP1; VEGF165R

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          UniProt ID: (Human) O14786, (Mouse) P97333

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          Entrez Gene ID: (Human) 8829, (Mouse) 18186

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          Function(s)
          vascular endothelial growth factor-activated receptor activity GTPase activator activity protein binding heparin binding coreceptor activity semaphorin receptor activity growth factor binding protein kinase binding cytokine binding vascular endothelial growth factor binding metal ion binding oxidoreductase
          Process(es)
          angiogenesis patterning of blood vessels vasculogenesis neural crest cell migration neuron migration positive regulation of endothelial cell proliferation sprouting angiogenesis cell migration involved in sprouting angiogenesis outflow tract septum morphogenesis substrate-dependent cell migration, cell extension signal transduction integrin-mediated signaling pathway cell-cell signaling nervous system development axon guidance axonal fasciculation heart development motor neuron axon guidance response to wounding organ morphogenesis positive regulation of endothelial cell migration positive regulation of smooth muscle cell migration dendrite development facial nerve structural organization trigeminal nerve morphogenesis trigeminal nerve structural organization vestibulocochlear nerve structural organization branchiomotor neuron axon guidance gonadotrophin-releasing hormone neuronal migration to the hypothalamus cell differentiation negative regulation of axon extension regulation of vascular endothelial growth factor receptor signaling pathway retinal ganglion cell axon guidance regulation of Cdc42 protein signal transduction cellular response to hepatocyte growth factor stimulus endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus ventral trunk neural crest cell migration vascular endothelial growth factor signaling pathway neuropilin signaling pathway VEGF-activated neuropilin signaling pathway positive regulation of phosphorylation negative regulation of neuron apoptotic process blood vessel endothelial cell migration endothelial cell migration positive regulation of angiogenesis viral entry into host cell platelet-derived growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway hepatocyte growth factor receptor signaling pathway sympathetic nervous system development neuron development axon extension regulation of axon extension involved in axon guidance positive regulation of axon extension involved in axon guidance negative regulation of axon extension involved in axon guidance artery morphogenesis axon extension involved in axon guidance positive chemotaxis positive regulation of filopodium assembly positive regulation of stress fiber assembly positive regulation of focal adhesion assembly axonogenesis involved in innervation regulation of vesicle-mediated transport dichotomous subdivision of terminal units involved in salivary gland branching angiogenesis involved in coronary vascular morphogenesis coronary artery morphogenesis retina vasculature development in camera-type eye retina vasculature morphogenesis in camera-type eye renal artery morphogenesis sympathetic ganglion development trigeminal ganglion development positive regulation of ERK1 and ERK2 cascade semaphorin-plexin signaling pathway commissural neuron axon guidance positive regulation of cell migration involved in sprouting angiogenesis endothelial tip cell fate specification sensory neuron axon guidance motor neuron migration sympathetic neuron projection extension sympathetic neuron projection guidance postsynapse organization regulation of plasma membrane bounded cell projection organization basal dendrite development basal dendrite arborization positive regulation of substrate adhesion-dependent cell spreading neural crest cell migration involved in autonomic nervous system development protein localization to early endosome facioacoustic ganglion development dorsal root ganglion morphogenesis otic placode development negative regulation of extrinsic apoptotic signaling pathway multicellular organism development cell migration peptidyl-tyrosine phosphorylation nerve development otic placode formation positive regulation of peptidyl-tyrosine phosphorylation cell migration involved in coronary vasculogenesis ganglion morphogenesis regulation of retinal ganglion cell axon guidance toxin transport semaphorin-plexin signaling pathway involved in neuron projection guidance semaphorin-plexin signaling pathway involved in axon guidance positive regulation of retinal ganglion cell axon guidance VEGF-activated neuropilin signaling pathway involved in axon guidance
          It has to be done as per old AB suggested Products section.

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