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Invitrogen
IRF1 Monoclonal Antibody (TK-1), eBioscience™
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FIGURE: 1 / 1
IRF1 Antibody (14-5836-82) in WB
Immunoblot of BALB/c bone marrow cells either stimulated for 16 hours with 1 µg/mL of LPS (lane 1) or unstimulated (lane 2). Lysates were analyzed by SDS-PAGE followed by immunoblotting with 2µg/mL of Anti-Mouse IRF1 Purified. Bands were visualized using Anti-Armenian Hamster IgG HRP.
Product Details
14-5836-82
Product Specifications
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
TK-1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The TK-1 monoclonal antibody reacts with mouse Interferon Regulatory Factor-1 (IRF-1). IRF-1 is a transcription factor that is expressed in all cells and has been shown to play many roles in the immune system. IRF-1 activates transcription of IFNgamma, and more recently has been shown to induce TNFalpha expression in response to IFNgamma. Recent studies in IRF-1 knockout mice have revealed additional pleiotropic effects of IRF-1. Impairment in IL-12 production by macrophages, impaired CD8+ T cell and NK cell maturation and the absence of Th1 responses with exclusive Th2 differentiation have all been reported in IRF-1 knockout mice. These IRF-1-deficient mice also have increased numbers of immature, tolerogenic dendritic cells and differentiated regulatory T cells.
Applications Reported: This TK-1 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This TK-1 antibody has been tested by immunoblot on mouse bone marrow cell lysates. This can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppression. Diseases associated with IRF1 include gastric cancer, somatic and lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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