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Invitrogen
Embigin Monoclonal Antibody (G7.43.1), eBioscience™
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FIGURE: 1 / 2
Embigin Antibody (14-5839-81) in WB
iScience 2024 -
Embigin is widely expressed until E10.5 and after that in specific organs (A) WT mouse embryos at embryonic days E8.5, E9.5, and E10.5 were stained with embigin and alpha-smooth muscle actin (alpha-SMA) antibodies by using the whole-mount immunostaining technique. As the negative control for embigin, only a secondary antibody was used; alpha-SMA was stained as a positive control, an asterisk indicates a dev... View More
Product Details
14-5839-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
G7.43.1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This G7.43.1 monoclonal antibody reacts with mouse embigin (gp70), a single transmembrane glycoprotein that is a member of the Ig superfamily similar to basigin and EMMPRIN. It contains two Ig domains thought to be involved in cellular adhesion and tissue remodeling. Expression of embigin is high before day 10 in fetal development. In the adult it is expressed on myeloid cells and T cells but not B cells. During hematopoeisis, embigin is expressed on HSCs and CLPs (common lymphoid progenitors) but absent on pro- and pre-B cells. In recent studies Pax 5 has been shown to repress embigin expression.
Applications Reported: This G7.43.1 antibody has been reported for use in flow cytometric analysis and immunohistology staining of frozen tissue sections.
Applications Tested: This G7.43.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Embigin is a 327 amino acid regulatory protein belonging to the immunoglobulin superfamily class of CAMs, and has two Ig-like (immunoglobulin-like) V- type domains. In situ hybridization analysis shows that Embigin has specific carbohydrate epitopes and plays an important role in early postimplantation embryogenesis. A highly glycosylated transmembrane glycoprotein, Embigin acts as a regulator of cell/ECM interactions during development and homeostasis of normal adult tissues. Transfection studies suggest its role in promotion of integrin-mediated cell-substratum adhesion. Widely expressed in most of the tissues and embryonal carcinoma cells, prostate and mammary cancer cell lines, it may also help in the classification of human carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Embigin; glycoprotein 70; Teratocarcinoma glycoprotein Gp-70
Gene Aliases: AL022799; Emb; Gp70
UniProt ID: (Mouse) P21995
Entrez Gene ID: (Mouse) 13723
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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