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Invitrogen
Nestin Monoclonal Antibody (Rat-401 (Rat401 (4D4))), eBioscience™
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FIGURE: 1 / 9
Nestin Antibody (14-5843-82) in ICC/IF
Immunofluorescence analysis of Nestin was performed using 70% confluent log phase PC-12 differentiated to neuronal cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Anti-Nestin Monoclonal Antibody (Rat-401 (Rat401 (4D4))), eBioscience™ (Product # 14-5843-82) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) High... View More
Product Details
14-5843-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
Rat-401 (Rat401 (4D4))
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody Rat-401 recognizes mouse and rat nestin, a Class VI intermediate filament protein (>170 kDa) primarily expressed in neuroepithelial stem cells. Upon neural differentiation, expression is down-regulated and within the adult nervous system is limited to progenitor cells in the cortical subventricular zone, the hippocampal dentate gyrus, dorsal root ganglia satellite cells, and a subpopulation of Schwann cells. Nestin expression is also present in other progenitor cell populations such as pancreatic islet, angiogenic endothelial cells, and bone marrow mesenchymal stem cells. Nestin filaments require vimentin and desmin for assembly into heterodimers and polymers. Upon differentiation, Nestin-containing filaments are replaced by cell type specific intermediate filament proteins, such as GFAP.
Applications Reported: This Rat-401 (Rat401 (4D4)) antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.
Applications Tested: This Rat-401 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells and by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ21841; intermediate filament protein; Nestin
Gene Aliases: AA166324; C78523; ESTM46; Nes
UniProt ID: (Mouse) Q6P5H2
Entrez Gene ID: (Rat) 25491, (Mouse) 18008
G2/M transition of mitotic cell cycle
nervous system development
brain development
response to ionizing radiation
neuron differentiation
positive regulation of intermediate filament depolymerization
embryonic camera-type eye development
response to nutrient levels
negative regulation of protein binding
response to drug
negative regulation of catalytic activity
negative regulation of neuron apoptotic process
cell projection morphogenesis
stem cell proliferation
positive regulation of neural precursor cell proliferation
multicellular organism development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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