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Invitrogen
TER-119 Monoclonal Antibody (TER-119), eBioscience™
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FIGURE: 1 / 48
TER-119 Antibody (14-5921-82) in Flow
Staining of C57BL/6 bone marrow cells with 0.25 µg of Rat IgG2b kappa Isotype Control Purified (Product # 14-4031-82) (open histogram) or 0.25 µg of Anti-Mouse TER-119 Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85).Cells in the erythrocyte region were used for analysis.
Product Details
14-5921-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
C. elegans,
Fish,
Human,
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
TER-119
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.
Applications Reported: The TER-119 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.
Applications Tested: The TER-119 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow. It is also present in yolk sac, and fetal and newborn liver. The TER-119 antigen is not expressed by cells of earlier erythroid development at BFU-e (blast-forming unit erythroid) stage or CFU-e (colony-forming unit erythroid) stage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ly-76; Ly76; TER119
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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