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Ubiquitin Monoclonal Antibody (eBioP4D1 (P4D1)), eBioscience™
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FIGURE: 1 / 5
Ubiquitin Antibody (14-6078-82) in WB
Western blot was performed using Ubiquitin Monoclonal Antibody (eBioP4D1 (P4D1)), eBioscience™ (Product # 14-6078-82) and ~80-260 kDa bands corresponding to poly ubiquitinated proteins were observed across the cell lines tested and increased upon treatment with MG132. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with 10 µM of MG132 for 90 minutes (Lane 2), HEK-293 (Lane 3) and HEK-293 treated with 10 µM of MG132 for 90 minutes (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX),... View More
Product Details
14-6078-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
All,
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Plant,
Yeast
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
eBioP4D1 (P4D1)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody eBioP4D1 (p4D1) recognizes ubiquitin, a small 8Ka protein. Ubiquitin units are linked to proteins via a ubiquitination process, thereby signaling the protein for degradation by the 26S proteosome. The process of ubiquitination plays a role in differentiation, cell cycle progression, apoptosis and disease progression. For example, neuronal inclusions called Lewis bodies from Parkinson patients are rich in ubiquitin.
Applications Reported: This eBioP4D1 (P4D1) antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.
Applications Tested: This eBioP4D1 (P4D1) antibody has been tested by immunblotting of treated cell lines. This can be used at less than or equal to 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 40S ribosomal protein S27-like; 40S ribosomal protein S27-like protein; 40S ribosomal protein S27a; 60S ribosomal protein L40; CEP52; Chap1; epididymis luminal protein 112; epididymis secretory protein Li 50; hPLIC-2; HRIHFB2157; HUBCEP52; OTTHUMP00000200786; OTTHUMP00000201692; PLIC-2; polyubiquitin B; polyubiquitin C; Polyubiquitin-B; Polyubiquitin-C; pro-ubiquitin; Ribosomal protein eS27-like; ribosomal protein S27; RPL40; RPS27A; rps27l; Small ribosomal subunit protein eS27-like; UBA52; UBB; UBC; Ubiquilin 2; ubiquitin; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein S27a; ubiquitin C; ubiquitin carboxyl extension protein 52; Ubiquitin carboxyl extension protein 80; ubiquitin precursor; ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-52 amino acid fusion protein; ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-ribosomal protein eL40 fusion protein; Ubiquitin-ribosomal protein eS31 fusion protein; ubiquitin/60S ribosomal fusion protein; unnamed protein product
Gene Aliases: 0610006J14Rik; 1810034D23Rik; 2700054O04Rik; AI194771; AL033289; CEP52; CEP80; D8Ertd21e; eS31; Gm1863; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; RPL40; RPS27A; RPS27L; S27A; TI-225; UBA52; UBA80; UBB; Ubb2; UBC; UBCEP1; UBCEP2; UBCEP80
RNA binding
structural constituent of ribosome
protein binding
zinc ion binding
protein tag
ubiquitin protein ligase binding
metal ion binding
translation activator activity
poly(A) RNA binding
cysteine-type endopeptidase activator activity involved in apoptotic process
protease binding
ribosomal protein
translational protein
cytoplasmic translation
translation
protein ubiquitination
modification-dependent protein catabolic process
ribosomal small subunit biogenesis
ribosomal small subunit assembly
DNA damage response, signal transduction by p53 class mediator
mitotic G1 DNA damage checkpoint
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of translation
biological_process
DNA repair
activation of cysteine-type endopeptidase activity involved in apoptotic process
cellular response to DNA damage stimulus
response to insecticide
protein modification process
male meiosis I
female meiosis I
male gonad development
female gonad development
hypothalamus gonadotrophin-releasing hormone neuron development
positive regulation of protein ubiquitination
regulation of neuron apoptotic process
mitochondrion transport along microtubule
neuron projection morphogenesis
regulation of mitochondrial membrane potential
adipose tissue development
fat pad development
regulation of proteasomal protein catabolic process
seminiferous tubule development
energy homeostasis
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
positive regulation of protein monoubiquitination
ubiquitin homeostasis
regulation of neuron death
ubiquitin-dependent protein catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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