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Invitrogen
PARP1 (cleaved Asp214) Monoclonal Antibody (HLNC4), eBioscience™
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FIGURE: 1 / 5
PARP1 (cleaved Asp214) Antibody (14-6668-80) in WB
Western blot was performed using Anti-PARP1 (cleaved Asp214) Monoclonal Antibody (HLNC4), eBioscience™(Product # 14-6668-82) and a 85 kDa band corresponding to PARP1 (cleaved Asp214) was observed across all cell lines. Nuclear enriched extracts (40 µg lysate) of Jurkat (Lane 1), Jurkat treated with Etoposide (1 µM for 16 hours) (Lane 2), HeLa (Lane 3), HeLa treated with Etoposide (1 µM for 16 hours) (Lane 4... View More
-WB-20170216130641.jpg?time=20240507)
-WB-20170216130641.jpg?time=20240507?time=20240507 "PARP1 (cleaved Asp214) Antibody (14-6668-80) in WB")
Product Details
14-6668-80
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
HLNC4
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
25 µg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: This HLNC4 monoclonal antibody reacts with human poly (ADP-ribose) polymerase (PARP1). This ubiquitous 116 kDa nuclear enzyme is involved in DNA repair. During apoptosis, active caspases -3, -6 and -7 cleave PARP1 after Asp214, thereby inactivating PARP1 and generating two apoptotic fragments sized 85 kDa and 25 kDa.
The HLNC4 antibody specifically recognizes the 85 kDa PARP1 fragment produced after cleavage and does not recognize the full-length 116 kDa protein.The following peptide was used as the immunogen: NH2-GVDEVAKKKSKKEKDC-COOH.
Applications Reported: This HLNC4 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This HLNC4 antibody has been tested by immunoblotting of staurosporine-treated Jurkat cells. This can be used at less than or equal to 0.1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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