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Invitrogen
IL-4 Monoclonal Antibody (8D4-8), eBioscience™
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FIGURE: 1 / 15
IL-4 Antibody (14-7049-81) in WB, Flow
Nucleic acids research 2015 -
Figure 6. piR30840 regulates the development of Th2 lymphocytes. ( A and B ) Human CD4 naive cells were cultured in a conditioned medium for Th2 development and then transfected with an s30840 (A) or piR30840 inhibitor (B). IL-4 expression was detected by qRT-PCR and western blotting analyses. P < 0.01. The data represents three independent experiments. ( C ) Detection of IL-4 expression in human PBMCs isol... View More
Product Details
14-7049-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Cynomolgus monkey,
Human,
Mouse,
Non-human primate,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
8D4-8
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.
Applications Reported:The 8D4-8 antibody has been reported for use in capture of human IL-4 by ELISA and in intracellular staining for flow cytometric analysis.
Applications Tested: The 8D4-8 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the biotinylated MP4-25D2 (Product # 13-7048-81) antibody for detection and recombinant human IL-4 (Product # 14-8049-80) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.
The fluorochrome-conjugated 8D4-8 antibody has been tested by intracellular staining for flow cytometric analysis. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation:Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; IL-4v2; IL4; ILN; Interleukin; interleukin 4; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra
Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4
UniProt ID: (Human) P05112
Entrez Gene ID: (Human) 3565
negative regulation of transcription from RNA polymerase II promoter
immune response
JAK-STAT cascade
cholesterol metabolic process
positive regulation of cell proliferation
positive regulation of gene expression
negative regulation of epithelial cell migration
positive regulation of macroautophagy
B cell differentiation
positive regulation of cell migration
positive regulation of B cell proliferation
negative regulation of tumor necrosis factor production
positive regulation of interleukin-10 production
positive regulation of interleukin-13 production
interleukin-4-mediated signaling pathway
type 2 immune response
positive regulation of T cell proliferation
T cell activation
B cell activation
macrophage activation
myeloid dendritic cell differentiation
negative regulation of apoptotic process
regulation of isotype switching
leukocyte activation
positive regulation of MHC class II biosynthetic process
positive regulation of T cell differentiation
negative regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of receptor-mediated endocytosis
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
negative regulation of inflammatory response
regulation of immune response
regulation of multicellular organismal process
regulation of primary metabolic process
dendritic cell differentiation
positive regulation of cold-induced thermogenesis
neuroinflammatory response
negative regulation of neuroinflammatory response
positive regulation of beta-amyloid clearance
positive regulation of cellular respiration
negative regulation of complement-dependent cytotoxicity
negative regulation of cellular response to transforming growth factor beta stimulus
negative regulation of endothelial cell apoptotic process
positive regulation of T-helper 2 cell cytokine production
positive regulation of ATP biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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