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Invitrogen
IL-1 alpha Monoclonal Antibody (364/3B3-14), eBioscience™
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FIGURE: 1 / 2
IL-1 alpha Antibody (14-7118-81) in Flow
The Journal of cell biology 2021 -
Figure 4. DOT1L overexpression (OE) increases H3K79me2/3 at the IL1A locus and is sufficient for SASP gene expression but does not affect other senescence phenotypes. IMR90 cells were infected with retrovirus-expressing human DOT1L or empty vector control. In some experiments, as a positive control, IMR90 cells were infected with retrovirus-expressing HRAS G12V (RAS) or empty vector control. (A) DOT1L mRNA ... View More
Product Details
14-7118-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
364/3B3-14
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 364/3B3-14 antibody reacts with human interleukin-1alpha.
Applications Reported: The 364/3B3-14 antibody has been reported for intracellular staining for flow cytometric analysis.
Applications Tested: This 364/3B3-14 antibody has been tested intracellular staining and flow cytometric analysis of stimulated human blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CTLA8; Hematopoietin-1; IL 1 a; IL 1α; IL-1 alpha; IL17; IL1A; IL1α; ILN; Interleukin; interleukin 17; Interleukin-1 alpha; Interleukin-17A; Interleukin1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha; RP23-160G19.8; unnamed protein product
Gene Aliases: IL-1 alpha; IL-1A; IL1; IL1-ALPHA; IL1A; IL1F1
UniProt ID: (Human) P01583
Entrez Gene ID: (Human) 3552
fever generation
positive regulation of cytokine production
connective tissue replacement involved in inflammatory response wound healing
cellular sodium ion homeostasis
apoptotic process
response to stress
inflammatory response
immune response
negative regulation of cell proliferation
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
cytokine-mediated signaling pathway
positive regulation of interleukin-2 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of immature T cell proliferation in thymus
cellular response to heat
ectopic germ cell programmed cell death
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of angiogenesis
positive regulation of mitotic nuclear division
positive regulation of transcription from RNA polymerase II promoter
response to copper ion
positive regulation of protein secretion
positive regulation of cell division
regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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