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Invitrogen
CX3CL1 (Fractalkine) Polyclonal Antibody, eBioscience™
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FIGURE: 1 / 6
CX3CL1 (Fractalkine) Antibody (14-7986-81) in WB
Western blot was performed using Anti-CX3CL1 (Fractalkine) Polyclonal Antibody, eBioscience™ (Product # 14-7986-81) and a 85 kDa band corresponding to soluble cleaved form of CX3CL1 (Fractalkine) was observed across tested cell line. Conditioned media (20 µg lysate) of HUVEC (Lane 1), HUVEC treated with TNF-alpha, 10 ng/mL for 12 hrs (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Pr... View More
Product Details
14-7986-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The rabbit polyclonal antibody reacts with recombinant and natural mouse, rat, and human fractalkine (CX3CL1); the antibody was generated using E. coli-expressed chemotactic domain of human CX3CL1 as an immunogen. CX3CL1, also known as neurotactin or fractalkine, is a membrane-bound CX3C chemokine. The mature protein is part of a 397-amino acid precursor and consists of a chemokine domain of 76 amino acids, a mucin stalk of 241 amino acids, a putative transmembrane domain of 18 amino acids, and an intracellular tail of 37 amino acids. Within the chemokine domain, the first two cysteine residues are separated by 3 amino acids (CX3C). CX3CL1 message is found in high abundance in the brain, kidney, lung and heart tissues. CX3CL1 is chemotactic for monocytes and other leukocytes including NK cells and may play a role in brain inflammation.
Applications Reported: This polyclonal has been reported for use in immunoblotting (WB), immunoprecipitation, and immunohistochemistry. Cross-reactivity to CX3CL1 of other species has not been determined.
Applications Tested: To achieve best results, titrate antibody by dilution for each application as follows:
Western Blot 1:1000-1:5000
Immunoprecipitation 1:300-1:800
Immunohistochemistry 1:100-1:500.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-a in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ABCD-3; C-X3-C motif chemokine 1; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; FK; Fractalkine; Neurotactin; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D, 1; Small-inducible cytokine D1; unnamed protein product
Gene Aliases: A-152E5.2; AB030188; ABCD-3; Acc1; AI848747; C3Xkine; CX3C; CX3CL1; CXC3; CXC3C; D8Bwg0439e; FKN; fractalkine; neurotactin; NTN; NTT; SCYD1
UniProt ID: (Human) P78423, (Rat) O55145, (Mouse) O35188
Entrez Gene ID: (Human) 6376, (Rat) 89808, (Mouse) 20312
microglial cell activation
positive regulation of cell-matrix adhesion
positive regulation of neuroblast proliferation
leukocyte migration involved in inflammatory response
response to ischemia
chemotaxis
defense response
inflammatory response
immune response
cell adhesion
G-protein coupled receptor signaling pathway
cell-cell signaling
positive regulation of cell proliferation
negative regulation of cell-substrate adhesion
positive regulation of neuron projection development
neuron remodeling
cytokine-mediated signaling pathway
positive regulation of cell migration
negative regulation of cell migration
neutrophil chemotaxis
leukocyte chemotaxis
regulation of lipopolysaccharide-mediated signaling pathway
positive regulation of actin filament bundle assembly
negative regulation of interleukin-1 alpha production
negative regulation of interleukin-1 beta production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
positive regulation of transforming growth factor beta1 production
integrin activation
autocrine signaling
wound healing
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
regulation of synaptic plasticity
eosinophil chemotaxis
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of smooth muscle cell proliferation
positive regulation of inflammatory response
regulation of neurogenesis
leukocyte adhesive activation
positive chemotaxis
positive regulation of calcium-independent cell-cell adhesion
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of protein kinase B signaling
angiogenesis involved in wound healing
cell chemotaxis
microglial cell proliferation
antimicrobial humoral immune response mediated by antimicrobial peptide
neuron cellular homeostasis
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
extrinsic apoptotic signaling pathway in absence of ligand
cell-cell adhesion
synapse pruning
negative regulation of hippocampal neuron apoptotic process
negative regulation of glutamate receptor signaling pathway
negative regulation of microglial cell activation
positive regulation of microglial cell migration
negative regulation of neuron migration
negative regulation of apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
response to hypoxia
aging
positive regulation of macrophage chemotaxis
platelet activation
positive regulation of cell adhesion
negative regulation of vasoconstriction
negative regulation of cytokine secretion
inhibitory postsynaptic potential
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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