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Invitrogen
Human IL-17A Recombinant Protein
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Product Details
14-8179-62
Product Specifications
Expression System
E. coli
Class
Recombinant
Type
Protein
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
5 µg
Storage conditions
-80°C
Shipping conditions
Dry ice
Product Specific Information
Description: Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A binds to a receptor that binds also to HVS13 (viral IL-17) and to CTLA-8.
This recombinant protein is E. coli expressed. Insect cell expressed Human IL-17A Recombinant Protein (cat. 14-8180) is also available.
Applications Reported: Human IL-17A Recombinant Protein is biologically active.
Applications Tested: The recombinant human IL-17A has been tested as the standard in a human IL-17A sandwich ELISA and in bioassay for induction of IL-6 production by NHDF cells. The ED50 is 0.5-5 ng/mL, corresponding to a specific activity of 2 x 10e6 - 2 x 10e5 Units/mg.
Source: E. coli expressed amino acids Ile 20-Ala 155 of mature human IL-17A accession # NM_002190.
Bioactivity: Measured by induction of IL-6 production by NHDF cells. The ED50 is 3.0 ng/mL, corresponding to a specific activity of 3.3 x 10e5 Units/mg.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.
Molecular Weight: The methionylated polypeptide has a predicted molecular mass of 15,666. The DTT reduced protein migrates as a 15 kDa polypeptide. The protein is a cystine linked homodimer and migrates as a 27 kDa protein on non-reducing SDS-PAGE.
Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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