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Invitrogen
SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), eBioscience™
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FIGURE: 1 / 2
SSEA3 Antibody (14-8833-80) in Flow
Staining of the 2102Ep cell line with 0.5 µg of Rat IgM, K Isotype Control Purified (Product # 14-4341-82) (open histogram) or 0.5 µg of Anti-Human/Mouse SSEA-3 Purified (filled histogram) followed by Anti-Rat IgM PE (Product # 12-0990-82).Total viable cells were used for analysis.
Product Details
14-8833-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rat
/ IgM
Class
Monoclonal
Type
Antibody
Clone
eBioMC-631 (MC-631)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation.
Applications Reported: This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This eBioMC-631 (MC-631) antibody has been tested by flow cytometric analysis of the 2102Ep cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: SSEA-3; stage-specific embryonic antigen 3
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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