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Invitrogen
CD321 (F11R) Monoclonal Antibody (WK9), eBioscience™
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FIGURE: 1 / 5
CD321 (F11R) Antibody (14-9321-82) in ICC/IF
Immunofluorescence analysis of CD321 was performed using 70% confluent log phase Caco-2 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD321 Monoclonal Antibody (Product # 14-9321-80) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Highl... View More
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-Flow-20170216130641.jpg?time=20240507?time=20240507 "CD321 (F11R) Antibody (14-9321-82) in Flow")
Product Details
14-9321-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
WK9
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins. CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, epithelial and endothelial cells, and plays a role in mediating cell-cell contact. CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin. CD321 is also involved in reovirus entry into cells.
Applications Reported: This WK9 antibody has been reported for use in flow cytometric analysis and immunoprecipitation. The WK9 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 38 kD under reducing and non-reducing conditions.
Applications Tested: This WK9 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins. CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, stem cells, epithelial and endothelial cells, and plays a role in mediating cell-cell contact. CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin. CD321 is involved in the maintenance of epithelial and endothelial barriers, hematopoiesis, angiogenesis, and tumor proliferation, invasion, and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD321; F11 R; F11R; immunoglobulin superfamily; JAM-1; JAM-1; platelet adhesion molecule PAM-1; JAM-A; Junctional adhesion molecule 1; Junctional adhesion molecule A; PAM-1; Platelet adhesion molecule 1; Platelet F11 receptor; platelet membrane glycoprotein; unnamed protein product
Gene Aliases: CD321; F11R; JAM; JAM1; JAMA; JCAM; KAT; PAM-1; UNQ264/PRO301
UniProt ID: (Human) Q9Y624
Entrez Gene ID: (Human) 50848
regulation of cytokine production
system process
inflammatory response
cell adhesion
leukocyte cell-cell adhesion
regulation of cell shape
cell differentiation
epithelial cell differentiation
actomyosin structure organization
regulation of actin cytoskeleton organization
positive regulation of Rho protein signal transduction
maintenance of blood-brain barrier
memory T cell extravasation
viral entry into host cell
intestinal absorption
leukocyte migration
regulation of cytoskeleton organization
negative regulation of stress fiber assembly
regulation of biological quality
cellular response to mechanical stimulus
protein localization to plasma membrane
establishment of endothelial intestinal barrier
regulation of membrane permeability
cell-cell adhesion
positive regulation of platelet aggregation
protein localization to bicellular tight junction
positive regulation of establishment of endothelial barrier
regulation of bicellular tight junction assembly
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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