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Invitrogen
Connexin 32 Monoclonal Antibody (Connexin32 (2A)), eBioscience™
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Product Details
14-9759-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
Connexin32 (2A)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The Connexin32 monoclonal antibody recognizes human, mouse, rat, and chicken Connexin 32. Connexins are a family of integral membrane proteins that form gap junction channels, which function to facilitate direct cell-cell communication. Gap junctions are comprised of two hemichannels, or connexons, which are made up of six connexin proteins. Over 20 connexin proteins have been identified and each combine to form either homomeric or heteromeric channels with different functional properties. Connexin 32 is expressed by myelin, neurons, oligodendrocytes, and hepatocytes, and plays a role in the development of ovarian follicles. Connexin 32 expression also plays a role in disease progression in chronic multiple sclerosis and experimental autoimmune encephalomyelitis. Mutations in the Connexin 32 gene, GJB1, result in a form of Charcot-Marie-Tooth disease which is characterized by peripheral nerve dysfunction.
Applications Reported: This Connexin32 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested: This Connexin32 antibody has been tested by immunohistochemistry of frozen mouse tissue and immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and the antibody can be used at less than or equal to 5 µg/mL. This Connexin32 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized human cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: connexin 32; Connexin-32; Cx32; GAP junction 28 kDa liver protein; Gap junction beta-1 protein; gap junction membrane channel protein beta 1; gap junction protein, beta 1, 32kDa; unnamed protein product
Gene Aliases: CMTX; CMTX1; Cnx32; CX32; Cxn-32; GJB1
UniProt ID: (Human) P08034, (Rat) P08033, (Mouse) P28230
Entrez Gene ID: (Human) 2705, (Chicken) 378797, (Rat) 29584, (Mouse) 14618
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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