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Invitrogen
Insulin Monoclonal Antibody (ICBTACLS), eBioscience™
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FIGURE: 1 / 5
Insulin Antibody (14-9769-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human pancreas using 0.25 µg/mL of Mouse IgG2a K Isotype Control Purified (left) or 0.25 µg/mL of Anti-Insulin Purified (right) followed by 10 µg/mL of Anti-Mouse IgG Biotin, Avidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-9769-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
ICBTACLS
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This ICBTACLS monoclonal antibody reacts with the hormone insulin, responsible for regulation of carbohydrate and fat metabolism. Blood glucose levels are maintained by insulin. Insulin secretion from pancreatic beta-cells within the islets of Langerhans results in uptake of excess glucose from the blood into organs and its storage in the form of glycogen. Patients with type 1 diabetes have an autoimmune destruction of insulin-producing beta-cells in the pancreas, resulting in insulin deficiency and elevated levels of blood glucose. Patients with type 2 diabetes have insulin resistance where insulin becomes less effective at reducing the amount of blood glucose. Insulin is also important for proper endothelial cell function in with respect to vascular elasticity in response to changing blood pressure and plays a role in cognition, learning, and memory.
The ICBTACLS antibody recognizes human, mouse, and rat insulin.
Applications Reported: This ICBTACLS antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.
Applications Tested: This ICBTACLS antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 0.25 µg/mL. This ICBTACLS antibody has been tested by western blot of non-reduced pancreatic tissue lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-peptide; Ins; INSU1; INSU2; INSU3B; INSUA; INSUB; INSUC; insulin splice variant; insulin variant; INS-splice; Insulin-1; preproinsulin; proinsulin; unnamed protein product
Gene Aliases: CP-II; IDDM; IDDM1; IDDM2; ILPR; Ins-1; Ins-2; Ins2-rs1; InsII; IRDN; Mody; MODY10; Mody4; PNDM4
UniProt ID: (Human) P01308, (Mouse) P01325, (Rat) P01322, (Rat) P01323, (Mouse) P01326
Entrez Gene ID: (Human) 3630, (Mouse) 16333, (Rat) 24505, (Rat) 24506, (Mouse) 16334
negative regulation of transcription from RNA polymerase II promoter
positive regulation of cytokine production
negative regulation of acute inflammatory response
glucose metabolic process
acute-phase response
ER overload response
G-protein coupled receptor signaling pathway
myoblast fusion
biological_process
positive regulation of cell proliferation
insulin receptor signaling pathway
lipid biosynthetic process
glycoprotein biosynthetic process
regulation of gene expression
positive regulation of gene expression
negative regulation of gene expression
positive regulation of glucose metabolic process
myotube differentiation
lipid catabolic process
lactate biosynthetic process
insulin processing
positive regulation of cell migration
regulation of protein localization
protein hexamerization
nitric oxide-cGMP-mediated signaling pathway
wound healing
lipoprotein biosynthetic process
negative regulation of protein catabolic process
vasodilation
regulation of phosphorylation
glucose homeostasis
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
negative regulation of gluconeogenesis
positive regulation of fatty acid biosynthetic process
positive regulation of glycogen biosynthetic process
positive regulation of DNA replication
negative regulation of glycogen catabolic process
positive regulation of glycolytic process
positive regulation of mitotic nuclear division
negative regulation of fatty acid metabolic process
positive regulation of glucose import
positive regulation of insulin receptor signaling pathway
alpha-beta T cell activation
regulation of protein secretion
negative regulation of protein secretion
positive regulation of protein secretion
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of lipid catabolic process
positive regulation of lipoprotein lipase activity
positive regulation of protein kinase B signaling
fatty acid homeostasis
relaxation of vascular smooth muscle
negative regulation of respiratory burst involved in inflammatory response
positive regulation of respiratory burst
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
positive regulation of ERK1 and ERK2 cascade
positive regulation of protein localization to nucleus
positive regulation of dendritic spine maintenance
regulation of protein localization to plasma membrane
negative regulation of reactive oxygen species biosynthetic process
neuron projection maintenance
negative regulation of feeding behavior
positive regulation of neuron projection development
response to cytokine
TORC1 signaling
response to peptide hormone
positive regulation of translation
response to cAMP
cellular response to glucose stimulus
cellular response to oxygen-containing compound
response to dimethyl sulfoxide
glucose transmembrane transport
negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
regulation of transcription, DNA-templated
cell-cell signaling
positive regulation of cell growth
positive regulation of cell differentiation
positive regulation of lipid biosynthetic process
regulation of synaptic plasticity
cognition
positive regulation of nitric-oxide synthase activity
positive regulation of peptide hormone secretion
positive regulation of brown fat cell differentiation
positive regulation of long-term synaptic potentiation
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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