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Invitrogen
HAI-1 Monoclonal Antibody (9B10), eBioscience™
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FIGURE: 1 / 1
HAI-1 Antibody (14-9960-82) in Flow
Staining of MCF7 cell line with 0.25 µg of Mouse IgG2a K Isotype Control Purified (Product # 14-4724-82) (open histogram) or 0.25 µg of Anti-Human HAI-1 Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Total viable cells were used for analysis.
Product Details
14-9960-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
9B10
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody 9B10 recognizes HAI-1 also known as hepatocyte growth factor activation inhibitor. HAI-1 is a single transmembrane protein of approximately 53 kDa with an extracellular Kunitz-type serine protease inhibitor domain. HAI-1 and HAI-2 are produced in membrane-associated forms, which are secreted as active, proteolytically truncated proteins. HAI can bind to and inhibit HGFA and matripase, which are responsible for converting HGF to an active form. Human HAI-1 transcript is expressed in various human tissues, such as adult placenta, kidney, pancreas, prostate, small intestine, fetal kidney and fetal lung. HAI-1 is a marker for prostate and breast cancers.
Applications Reported: This 9B10 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB) under nonreducing conditions only.
Applications Tested: This 9B10 antibody has been tested by flow cytometric analysis of human cell line MCF7. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HAI-1; HAI-1B; Hepatocyte growth factor activator inhibitor type 1; Kunitz-type protease inhibitor 1; serine protease inhibitor, Kunitz type 1; unnamed protein product
Gene Aliases: HAI; HAI1; MANSC2; SPINT1; UNQ223/PRO256
UniProt ID: (Human) O43278
Entrez Gene ID: (Human) 6692
neural tube closure
embryonic placenta development
epidermis development
extracellular matrix organization
positive regulation of glial cell differentiation
epithelium development
branching involved in labyrinthine layer morphogenesis
placenta blood vessel development
cellular response to BMP stimulus
negative regulation of neural precursor cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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