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Invitrogen
CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), Functional Grade, eBioscience™
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-Functional-Grade-Flow-20170216130641.jpg?time=20240507 "CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)")
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-Functional-Grade-Flow-20170216130641.jpg?time=20240507?time=20240507 "CD54 (ICAM-1) Antibody (16-0541-81) in Flow")
Product Details
16-0541-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
YN1/1.7.4
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody.
Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis. YN1/1.7.4 has also been reported in blocking of CD54 in functional studies.
Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells
Gene Aliases: CD54; Icam-1; Icam1; Ly-47; MALA-2
UniProt ID: (Mouse) P13597
Entrez Gene ID: (Mouse) 15894
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
acute inflammatory response to antigenic stimulus
T cell antigen processing and presentation
positive regulation of cellular extravasation
cell adhesion
leukocyte cell-cell adhesion
sensory perception of sound
regulation of cell shape
single organismal cell-cell adhesion
regulation of cell adhesion
positive regulation of actin filament polymerization
cell adhesion mediated by integrin
positive regulation of GTPase activity
adhesion of symbiont to host
positive regulation of nitric oxide biosynthetic process
positive regulation of vasoconstriction
receptor-mediated virion attachment to host cell
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of NF-kappaB transcription factor activity
negative regulation of calcium ion transport
establishment of endothelial barrier
positive regulation of ERK1 and ERK2 cascade
cellular response to glucose stimulus
establishment of Sertoli cell barrier
regulation of ruffle assembly
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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