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Invitrogen
NKG2A/C/E Monoclonal Antibody (20d5), Functional Grade, eBioscience™
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FIGURE: 1 / 4
NKG2A/C/E Antibody (16-5896-85) in Flow
Staining of C57Bl/6 splenocytes with 0.5 µg of Rat IgG2a Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.125 µg of Anti-Mouse NKG2A/C/E Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Total viable cells were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
16-5896-85
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
20d5
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 20d5 monoclonal antibody reacts with the mouse NKG2A, C, and E. NKG2 molecules belong to a C-type lectin-like family of cell surface receptors expressed by mouse NK and NKT cell lineages. NKG2 molecules form heterodimeric complexes with CD94 and are responsible for recognition of non-classical MHC class I antigen Qa-1.
When co-staining with NKG2A^B6 clone 16a11, it is important to stain for NKG2A-B6 first then subsequently with NKG2A/C/E, as steric hindrance has been observed if co-stained concurrently.
Applications Reported: This 20d5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 20d5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. The protein encoded by this gene belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells. This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells. The genes of NKG2 family members form a killer cell lectin-like receptor gene cluster on chromosome 12. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-type lectin; cd159c; cd159e; Cluster of Differentiation 159a; killer cell lectin-like receptor subfamily C, member 2; natural killer cell group 2E cell receptor; natural killer group 2A; NKG2; type II integral membrane protein; C-type lectin superfamily member; unnamed protein product
Gene Aliases: CD159a; Klrc2; NKG2A; NKG2B; NKG2C; Nkg2e
Entrez Gene ID: (Mouse) 16641, (Mouse) 16642, (Mouse) 58179
stimulatory C-type lectin receptor signaling pathway
natural killer cell mediated immunity
immune system process
immune response-regulating cell surface receptor signaling pathway
regulation of natural killer cell activation
regulation of natural killer cell mediated cytotoxicity
positive regulation of natural killer cell mediated cytotoxicity
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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