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Invitrogen
CD200 Receptor Monoclonal Antibody (OX108), Functional Grade, eBioscience™
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FIGURE: 1 / 2
CD200 Receptor Antibody (16-9201-82) in Flow
PloS one 2021 -
10.1371/journal.pone.0244770.g003 Fig 3 U937-CD200R mutants express CD200R on their surface. A) Conserved residues of CD200R in mammals were mutated as indicated. B) U937 cells were transduced with different CD200R mutants and tested for their CD200R surface expression by flow cytometry. Representative figures of the gating strategy are shown. First the population of live cells was selected. From these live... View More
Product Details
16-9201-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OX108
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody OX108 recognizes human CD200R also known as OX2. CD200R is an inhibitory receptor with a similar structure as its ligand, CD200, yet lacks an ITIM domain typically found in inhibitory receptors. Instead the CD200R cytoplasmic domain contains a novel phosphotyrosine binding domain, NPXY, which after binding SHIP inhibits ERK, JNK and MAPK p38 pathways. In activated macrophages signaling results in inhibition of TNFalpha secretion. Isoforms of CD200R have been identified and are thought to play a major role in differentiation, especially in regards to tolerogenic DCs.
Expression is restricted to hematopoietic cells: myeloid cells (monocytes, macrophages, DCs, neutrophils, mast cells and basophils) and a subset of T lymphocytes as well as langerhans (LC) cells and dendritic epidermal T cells. The epitope of OX108 is thought to be near the binding site of CD200.
Applications Reported: This OX108 antibody has been reported for use in flow cytometric analysis and functional assays.
Applications Tested: This OX108 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
CD200R (OX2R) is a receptor for the OX-2 membrane glycoprotein, and both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. CD200R is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. In mouse, CD200R is expressed primarily by peripheral blood monocytes and neutophils but also by other leucocytes including T-lymphocytes and mast cells. Further mouse studies of a gene related to CD200R suggests that the receptor may control myeloid function in a tissue-specific manner. Alternative splicing of CD200R results in multiple transcript variants. Diseases associated with CD200R dysfunction include primary hypertrophic osteoarthropathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD200 cell surface glycoprotein receptor; CD200 R1; Cell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; HuCD200R1; MOX2 receptor; unnamed protein product
Gene Aliases: CD200R; CD200R1; CRTR2; HCRTR2; MOX2R; OX2R; UNQ2522/PRO6015
UniProt ID: (Human) Q8TD46
Entrez Gene ID: (Human) 131450
signal transduction
negative regulation of interleukin-6 production
heterotypic cell-cell adhesion
intracellular signal transduction
Fc receptor signaling pathway
regulation of neuroinflammatory response
negative regulation of neuroinflammatory response
negative regulation of macrophage migration
negative regulation of T cell migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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