CD1c Monoclonal Antibody (L161), APC, eBioscience™

Product Specific Information

Description: This L161 monoclonal antibody detects CD1c (also known as BDCA-1), a glycoprotein that is noncovalently linked to beta-2 microglobulin on thymocytes and antigen presenting cells such as dendritic and Langerhans cells. This molecule is also expressed on some circulating and marginal zone B cells, as well as in lymph nodes and germinal centers. CD1c is involved in the presentation of lipid antigens such as microbial fatty acids to effector T cells during the adaptive immune response. Finally, alternative splicing gives rise to three different isoforms of CD1c (soluble, membrane, and cytoplasmic/soluble isoforms).

Applications Reported: This L161 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This L161 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD1c is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. This family of proteins is involved in the presentation of lipid and glycolipid antigens, both of self and microbial origin, to T cells during the adaptive immune response. CD1c is expressed on some circulating and marginal zone B cells, as well as in lymph nodes and germinal centers. It plays a crucial role in presenting lipid antigens, such as microbial fatty acids, to effector T cells. The protein encoded by the CD1c gene localizes to late endosomes and lysosomes, utilizing a tyrosine-based motif in its cytoplasmic tail for targeting. Vesicular acidification is required for CD1c to bind lipid antigens effectively. The human genome contains five CD1 family genes organized in a cluster on chromosome 1, with each member differing in cellular localization and specificity for particular lipid ligands. CD1c undergoes alternative splicing, resulting in three different isoforms: soluble, membrane-bound, and cytoplasmic/soluble isoforms, highlighting its functional diversity in immune processes.