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Invitrogen
PLZF Monoclonal Antibody (9E12), APC, eBioscience™
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FIGURE: 1 / 1
PLZF Antibody (17-9322-82) in Flow
LEFT: C57BL/6 thymocytes were surface stained with Anti-Mouse NK1.1 PE-Cyanine7 (Product # 25-5941-82) and Anti-Mouse TCR beta FITC (Product # 11-5961-82) followed by intracellular staining with 0.06 µg of Anti-Human/Mouse PLZF APC using the Foxp3/Transcription Factor Staining Buffer Set and protocol (Product # 00-5523-00). Viable, TCR beta+ cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis. RIGHT: Normal human peripheral blood cells were surface stained with Anti-Human CD56 (NCAM) FIT... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
17-9322-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
9E12
Immunogen
PLFZ peptide
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This 9E12 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans. In mice, PLZF is highly expressed in immature CD1d-resricted NKT2 and NKT1 cells, and a subset of gamma delta (Vg1.1+Vd6.3+) T cells. Studies have also demonstrated expression of PLZF in non-invariant CD1d-restricted T cells, as well as non-CD1d-restricted innate T cells. In humans, PLZF is expressed in NK cells, gamma delta T cells, as well as CD4 and CD8+ T cells. PLZF is also expressed in MR1-specific mucosal-associated invariant T cells, as well as in MHC Class II-restricted T cells that develop via a thymocyte-thymocyte interaction in humans. PLZF is expressed by innate lymphoid cells and its progenitor cells. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells, NK cell function, cellular quiescence, growth suppression, and maintenance of homeostatis. Finally, PLZF has been shown to inhibit gene expression induced by retinoic acid receptor.
This 9E12 antibody stains mouse thymocytes brighter than Mags.21F7.
This 9E12 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Green's luxoid; promyelocytic leukaemia zinc finger; unnamed protein product; zinc finger protein 145; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); zinc finger protein PLZF
Gene Aliases: lu; PLZF; Zfp145; ZNF145
UniProt ID: (Human) Q05516
Entrez Gene ID: (Human) 7704, (Mouse) 235320
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription corepressor binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
transcription cofactor activity
protein binding
protein domain specific binding
type 2 angiotensin receptor binding
identical protein binding
protein homodimerization activity
RNA polymerase II transcription factor activity, sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
mesonephros development
tolerance induction in gut-associated lymphoid tissue
regulation of transcription from RNA polymerase II promoter
apoptotic process
central nervous system development
regulation of gene expression
protein ubiquitination
hemopoiesis
myeloid cell differentiation
positive regulation of chondrocyte differentiation
protein localization to nucleus
embryonic hindlimb morphogenesis
embryonic digit morphogenesis
ossification involved in bone maturation
regulatory T cell differentiation
regulation of cell differentiation
positive regulation of fat cell differentiation
negative regulation of myeloid cell differentiation
positive regulation of ossification
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of NK T cell differentiation
cartilage development
positive regulation of multicellular organismal process
positive regulation of cartilage development
regulation of multicellular organismal development
skeletal system development
regulation of transcription, DNA-templated
negative regulation of cell proliferation
embryonic pattern specification
anterior/posterior pattern specification
forelimb morphogenesis
hindlimb morphogenesis
positive regulation of apoptotic process
male germ-line stem cell asymmetric division
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