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AKT3 Monoclonal Antibody (9A5.H9.G7)
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FIGURE: 1 / 3
AKT3 Antibody (200-301-J36) in ICC/IF
Immunofluorescence of Mouse monoclonal anti-AKT3 antibody. Cell Type: A431 cells. Fixation: 4% paraformaldehyde 10 min. Permeabilization: 0.5% Triton X 30 min. Primary Ab: 200-301-J36 1:250 72 hours 4°C. Secondary Ab: 610-142-121 1:1000 Overnight 4°C.
Product Details
200-301-J36
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
9A5.H9.G7
Immunogen
Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
Target Information
AKT3 (PKB gamma) has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). It is involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, glycogen synthesis and glucose uptake. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZP434N0250; PKB gamma; PKBgamma/Akt3; PKBgamma1; Protein kinase Akt-3; Protein kinase B gamma; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; similar to rat serine-threonine protein kinase; STK-2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Gene Aliases: AKT3; MPPH; MPPH2; PKB-GAMMA; PKBG; PRKBG; RAC-gamma; RAC-PK-gamma; STK-2
UniProt ID: (Human) Q9Y243
Entrez Gene ID: (Human) 10000
mitochondrial genome maintenance
positive regulation of endothelial cell proliferation
protein phosphorylation
signal transduction
insulin receptor signaling pathway
positive regulation of cell migration
positive regulation of TOR signaling
intracellular signal transduction
negative regulation of apoptotic process
positive regulation of blood vessel endothelial cell migration
positive regulation of angiogenesis
positive regulation of cell size
brain morphogenesis
homeostasis of number of cells within a tissue
positive regulation of cell migration involved in sprouting angiogenesis
negative regulation of PERK-mediated unfolded protein response
positive regulation of vascular endothelial cell proliferation
positive regulation of artery morphogenesis
negative regulation of cellular senescence
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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