Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Western Blot Products
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Food and Beverage
    • Lab Solutions
    • Pharma and Biopharma
    • Real-Time PCR
    • Semiconductor Analysis
    • Clinical and Diagnostics
    • Digital Solutions
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • See all services
  • Help and Support
    • Order Help
    • Digital Solutions
    • Product Support
    • Technical Information
    • Training and Education
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Who We Serve
    • Biotech
    • Biopharma
    • CDMO
    • Lab Diagnostics
    • Industrial and Applied Sciences
  • Special Offers
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search

Search All
Search button
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Custom Products & Projects
            • Instrument Management
          • Primary Antibodies ›
          • AKT1 Antibodies

          Rockland

          AKT1 Monoclonal Antibody (14E5.A2.B2.H9), FITC

          View all (184) AKT1 antibodies

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support

          Cite AKT1 Monoclonal Antibody (14E5.A2.B2.H9), FITC

          Additional Information:
          {{banner.description}}
          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

          Product Details

          200-302-I51

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          -

          Immunohistochemistry (IHC)

          Assay-dependent
          -

          ELISA (ELISA)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          14E5.A2.B2.H9

          Immunogen

          Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development.

          Conjugate

          FITC FITC FITC
          • Unconjugated
          • APC 
          • Biotin 
          • PE 
          • Request custom conjugation

          Excitation/Emission Max

          498/517 nm View spectra spectra

          Form

          Lyophilized

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          0.02M potassium phosphate, pH 7.2, with 0.5M NaCl, 10mg/mL BSA

          Contains

          0.01% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

          Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.

          Target Information

          AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

          Have you cited this product in a publication?
          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; C-AKT; CAKT; murine thymoma viral (v-akt) oncogene homolog 1; PKB; PKB alpha; PKB beta; PKB gamma; PKBG; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; unnamed protein product; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1

          View more View less

          Gene Aliases: AKT; AKT1; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA

          View more View less

          UniProt ID: (Human) P31749, (Mouse) P31750, (Rat) P47196

          View more View less

          Entrez Gene ID: (Human) 207, (Mouse) 11651, (Rat) 24185

          View more View less

          Function(s)
          nucleotide binding protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding calmodulin binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity transferase activity enzyme binding kinase binding protein kinase binding nitric-oxide synthase regulator activity protein serine/threonine kinase inhibitor activity identical protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding 14-3-3 protein binding potassium channel activator activity protein serine kinase activity TORC2 complex binding protein kinase C binding transferase activity, transferring phosphorus-containing groups protein phosphatase 2A binding non-receptor serine/threonine protein kinase
          Process(es)
          osteoblast differentiation maternal placenta development positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis cytoplasmic translation complement receptor mediated signaling pathway sphingosine-1-phosphate signaling pathway glycogen metabolic process glycogen biosynthetic process regulation of glycogen biosynthetic process glucose metabolic process gluconeogenesis translational initiation regulation of translation protein phosphorylation ubiquitin-dependent protein catabolic process protein import into nucleus NLS-bearing protein import into nucleus nitric oxide biosynthetic process ER to Golgi vesicle-mediated transport Golgi to plasma membrane transport apoptotic process activation-induced cell death of T cells inflammatory response response to oxidative stress signal transduction epidermal growth factor receptor signaling pathway G-protein coupled receptor signaling pathway germ cell development nervous system development cell proliferation insulin receptor signaling pathway apoptotic mitochondrial changes cellular response to starvation response to heat response to hormone gene expression negative regulation of autophagy positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport fibroblast migration positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of glucose metabolic process regulation of neuron projection development negative regulation of macroautophagy phosphorylation cell migration protein ubiquitination peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cytokine-mediated signaling pathway oogenesis stage cell differentiation protein catabolic process positive regulation of cell growth regulation of cell migration positive regulation of cell migration negative regulation of cell migration anaphase-promoting complex-dependent catabolic process SCF-dependent proteasomal ubiquitin-dependent protein catabolic process T cell costimulation negative regulation of protein ubiquitination regulation of myelination lipopolysaccharide-mediated signaling pathway cellular response to nutrient levels TOR signaling negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity response to food peripheral nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus regulation of protein localization positive regulation of peptidyl-serine phosphorylation cellular response to stress negative regulation of toll-like receptor signaling pathway positive regulation of toll-like receptor signaling pathway toll-like receptor 4 signaling pathway cellular response to amino acid starvation response to fluid shear stress protein K29-linked ubiquitination intracellular signal transduction interleukin-18-mediated signaling pathway cellular response to vascular endothelial growth factor stimulus cellular response to decreased oxygen levels SREBP-SCAP complex retention in endoplasmic reticulum tRNA stabilization calcium ion transmembrane import into mitochondrion PERK-mediated unfolded protein response TORC1 signaling glucose homeostasis regulation of apoptotic process Golgi to plasma membrane protein transport negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process anoikis regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of cholesterol biosynthetic process positive regulation of fat cell differentiation positive regulation of cilium assembly positive regulation of glycogen biosynthetic process negative regulation of Notch signaling pathway negative regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter negative regulation of translational initiation positive regulation of translational initiation nitric oxide metabolic process positive regulation of glucose import positive regulation of organ growth protein autophosphorylation positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway behavioral response to pain positive regulation of smooth muscle cell proliferation striated muscle cell differentiation positive regulation of protein metabolic process protein maturation excitatory postsynaptic potential response to growth hormone regulation of vesicle-mediated transport mammary gland epithelial cell differentiation labyrinthine layer blood vessel development protein localization to cilium response to UV-A response to growth factor protein localization to endoplasmic reticulum protein K11-linked ubiquitination cellular response to calcium ion cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus tricarboxylic acid metabolic process establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion cellular response to rapamycin protein K6-linked ubiquitination negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis vascular endothelial cell response to laminar fluid shear stress regulation of postsynapse organization tRNA (guanine-N7)-methylation regulation of tRNA methylation cellular response to oxidised low-density lipoprotein particle stimulus cGAS/STING signaling pathway negative regulation of protein localization to lysosome negative regulation of cGAS/STING signaling pathway beta-arrestin-dependent dopamine receptor signaling pathway positive regulation of G1/S transition of mitotic cell cycle positive regulation of protein localization to nucleus cellular response to peptide regulation of signal transduction by p53 class mediator negative regulation of cilium assembly negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of leukocyte cell-cell adhesion positive regulation of protein localization to plasma membrane negative regulation of protein maturation negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway negative regulation of PERK-mediated unfolded protein response negative regulation of TORC1 signaling positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of protein localization to endoplasmic reticulum positive regulation of anaphase-promoting complex-dependent catabolic process cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus positive regulation of protein localization to cell surface regulation of type B pancreatic cell development negative regulation of lymphocyte migration negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway protein import into nucleus, translocation positive regulation of protein phosphorylation carbohydrate metabolic process translation negative regulation of protein kinase activity transport cytoskeleton organization multicellular organism development aging carbohydrate transport negative regulation of endopeptidase activity glucose transport cell projection organization positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle positive regulation of cellular protein metabolic process anagen positive regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of cell size positive regulation of vasoconstriction negative regulation of JNK cascade positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity glycogen cell differentiation involved in embryonic placenta development cellular response to mechanical stimulus cellular response to organic cyclic compound cellular response to hypoxia positive regulation of establishment of protein localization to plasma membrane hyaluronan metabolic process
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us About Us
          • Careers Careers
          • Investors Investors
          • News News
          • Social Responsibility Social Responsibility
          • Trademarks
          • Policies and Notices
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Policy
          • Price & Freight Policy
          © 2006-2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          Taiwan flag icon
          Taiwan

          Your items have has been added!


          Host server : magellan-search-green-7d94cb4b65-4lm45:80/100.66.75.98:80.
          git-commit: c9e08c96761173abe34e68f880379696776a4827
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.44.1-2026.02.97.1.0