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AKT2 Monoclonal Antibody (16G11.E8)
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FIGURE: 1 / 4
AKT2 Antibody (200-501-E71) in IHC (P)
Immunohistochemistry of Rat monoclonal anti AKT2 Antibody in human breast carcinoma. Tissue: Human Breast Cancer. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. antibody: anti-AKT2 at 2 µg/mL for 1.5 hour @ room temp. Secondary Ab: mouse anti-rat at 1:50 for 458221 RT.
Product Details
200-501-E71
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
16G11.E8
Immunogen
Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.
Target Information
AKT2 is a phosphoinositide-dependent serine/threonine protein kinase that is enriched in insulin responsive tissues and has been implicated in the metabolic actions of the hormone. Akt is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: murine thymoma viral (v-akt) homolog-2; PKB beta; Protein kinase Akt-2; Protein kinase B beta; putative v-akt murine thymoma viral oncoprotein 2; RAC protein kinase beta; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; unnamed protein product; v-akt murine thymoma viral oncogene homolog 2
Gene Aliases: AKT2; HIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA
UniProt ID: (Human) P31751
Entrez Gene ID: (Human) 208
glycogen metabolic process
glycogen biosynthetic process
glucose metabolic process
regulation of translation
apoptotic process
signal transduction
insulin receptor signaling pathway
negative regulation of plasma membrane long-chain fatty acid transport
positive regulation of glucose metabolic process
regulation of cell migration
positive regulation of cell migration
positive regulation of fatty acid beta-oxidation
peripheral nervous system myelin maintenance
cellular response to insulin stimulus
intracellular signal transduction
protein modification process
negative regulation of apoptotic process
positive regulation of blood vessel endothelial cell migration
fat cell differentiation
positive regulation of glycogen biosynthetic process
positive regulation of glucose import
protein stabilization
regulation of cell cycle
mammary gland epithelial cell differentiation
cellular response to high light intensity
protein localization to plasma membrane
positive regulation of protein targeting to membrane
retinal rod cell apoptotic process
positive regulation of cap-dependent translational initiation
negative regulation of PERK-mediated unfolded protein response
positive regulation of cell motility
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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