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Mouse Hematopoietic Lineage Antibody Cocktail, FITC, eBioscience™
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FIGURE: 1 / 2
Mouse Hematopoietic Lineage Antibody (22-7770-72) in Flow
C57Bl/6 bone marrow cells were stained with the Mouse Hematopoietic Lineage FITC Cocktail, Anti-Mouse CD117 PE (Product # 12-1171-82) and Anti-Mouse CD34 eFluor® 660 (Product # 50-0341-82). Total viable cells, as determined by 7-AAD (Product # 00-6993-50), were used for analysis. Events displayed on the right plot are gated on lineage negative events, as shown on the left histogram.
Product Details
22-7770-72
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
Class
Cocktail
Type
Antibody
Clone
17A2, RA3-6B2, M1/70, TER-119, RB6-8C5
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
20 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The Mouse Hematopoietic Lineage FITC Cocktails contains FITC-conjugated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on the expression of cell surface antigens.
This cocktail contains the following antibodies:
Anti-Mouse CD3 (17A2) FITC
Anti-Mouse CD45R (B220) (RA3-6B2) FITC
Anti-Mouse CD11b (M1/70) FITC
Anti-Mouse TER-119 (TER-119) FITC
Anti-Mouse Ly-G6 (Gr-1) (RB6-8C5) FITC
Applications Reported: The Mouse Hematopoietic Lineage FITC Cocktail has been reported for use in flow cytometric analysis.
Applications Tested: The Mouse Hematopoietic Lineage FITC Cocktail has been pre-titrated and tested by flow cytometric analysis of C57Bl/6 bone marrow cells. This can be used at 20 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Target Information
Hematopoietic Lineage antibodies can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: 17A2; Hematopoietic Lineage Cocktail; M1/70; RA3-6B2; RB6-8C5; TER-119
Disclaimer
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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