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Invitrogen
GM-CSF Monoclonal Antibody (MP1-22E9), PE-Cyanine7, eBioscience™
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FIGURE: 1 / 2
GM-CSF Antibody (25-7331-82) in Flow
eBioscience™ Mouse Cytokine Positive Control Cells (Product # 00-4500-51) were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD4 Monoclonal Antibody, FITC (Product # 11-0042-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, PE-Cy7 (Product # 25-4321-81) (left) or 0.125 µg of GM-CSF Monoclonal Antibody, PE-Cy7 (right). Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
25-7331-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MP1-22E9
Conjugate
PE-Cyanine7
PE-Cyanine7
PE-Cyanine7
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. mouse GM-CSF is a 14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.
Applications Reported: This MP1-22E9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This MP1-22E9 antibody has been tested by intracellular staining followed by flow cytometric analysis of restimulated mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: colony-stimulating factor; granulocyte macrophage colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; granulocyte/macrophage colony-stimulating factor; M-GM-CSF; MGC131935; MGC138897; MGC151255; MGC151257; put. GM-CSF; unnamed protein product
Gene Aliases: CSF; Csfgm; Gm-CSf; GMCSF; MGI-IGM
UniProt ID: (Mouse) P01587
Entrez Gene ID: (Mouse) 12981
histamine secretion
embryonic placenta development
immune response
JAK-STAT cascade
positive regulation of cell proliferation
regulation of gene expression
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
myeloid cell differentiation
neutrophil differentiation
monocyte differentiation
macrophage differentiation
positive regulation of cell migration
positive regulation of interleukin-23 production
granulocyte-macrophage colony-stimulating factor signaling pathway
epithelial fluid transport
regulation of cell proliferation
myeloid dendritic cell differentiation
regulation of circadian sleep/wake cycle, sleep
negative regulation of transcription, DNA-templated
positive regulation of leukocyte proliferation
positive regulation of podosome assembly
cellular response to granulocyte macrophage colony-stimulating factor stimulus
dendritic cell differentiation
receptor signaling pathway via STAT
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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