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Invitrogen

Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803), PE-Cyanine7, eBioscience™

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1 Reference
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Product Details

25-9008-41

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test
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Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

KIKSI0803

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815117

Product Specific Information

Description: This KIKSI0803 monoclonal antibody recognizes signal transducer and activator of transcription 1 (STAT1) when phosphorylated on tyrosine 701. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. Phosphorylation of STAT1 on tyrosine 701 by JAK1 and JAK2 is essential for STAT1 dimer formation, nuclear translocation, and DNA binding activity. In response to IFN gamma stimulation, STAT1 homodimerizes or forms heterodimers with STAT3 that can bind to GAS (IFN gamma-activated sequence) promoter elements. In response to either IFN alpha or IFN beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind ISRE (IFN-stimulated response element) promoter elements.

Specificity of this KIKSI0803 clone was determined by ELISA, flow cytometry, and western blotting.

Applications Reported: This KIKSI0803 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This KIKSI0803 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using Protocol C: Two-step protocol for Fixation/Methanol. Protocol A: Two-step protocol for intracellular (cytoplasmic) proteins and Protocol B: One-step protocol for intracellular (nuclear) proteins cannot be used. All Protocols can be found in “Staining Intracellular Antigens for Flow Cytometry" located at www.thermofisher.com/flowprotocols . This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: DKFZp686B04100; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; STAT; STAT 1; STAT-1; stat1 alpha; transcription factor; Transcription factor ISGF-3 components p91/p84; transcription factor; 91 kDa; unnamed protein product

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Gene Aliases: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91

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UniProt ID: (Human) P42224

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Entrez Gene ID: (Human) 6772

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding tumor necrosis factor receptor binding protein binding enzyme binding CCR5 chemokine receptor binding histone acetyltransferase binding histone binding identical protein binding protein homodimerization activity sequence-specific DNA binding ubiquitin-like protein ligase binding cadherin binding protein phosphatase 2A binding promoter-specific chromatin binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter negative regulation of endothelial cell proliferation positive regulation of mesenchymal cell proliferation positive regulation of defense response to virus by host negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response signal transduction JAK-STAT cascade response to nutrient blood circulation positive regulation of cell proliferation response to xenobiotic stimulus response to mechanical stimulus negative regulation of angiogenesis positive regulation of interferon-alpha production cellular response to insulin stimulus tumor necrosis factor-mediated signaling pathway response to cytokine response to interferon-gamma response to interferon-beta cellular response to interferon-beta interleukin-7-mediated signaling pathway interleukin-9-mediated signaling pathway regulation of cell proliferation response to hydrogen peroxide regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling response to peptide hormone endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of erythrocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade negative regulation by virus of viral protein levels in host cell positive regulation of smooth muscle cell proliferation negative regulation of developmental process response to cAMP defense response to virus interferon-gamma-mediated signaling pathway type I interferon signaling pathway renal tubule development interleukin-27-mediated signaling pathway cellular response to cytokine stimulus cellular response to interferon-gamma metanephric mesenchymal cell proliferation involved in metanephros development metanephric mesenchymal cell differentiation negative regulation of metanephric nephron tubule epithelial cell differentiation receptor signaling pathway via STAT
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