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Invitrogen
Goat anti-Human IgG (H+L) Secondary Antibody, Biotin
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Product Details
31770
Applications
Tested Dilution
Western Blot (WB)
1:200
Immunohistochemistry (IHC)
1:200
Immunocytochemistry (ICC/IF)
1:200
ELISA (ELISA)
1:200
In Situ Hybridization (ISH)
1:200
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Amount
1.5 mg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.8, with 3mg/mL BSA
Contains
0.08% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Product # 31770 has been successfully used in Western blot, IF, ICC, IHC and ISH applications.
Product # 31770 reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins, but does not react against non-immunoglobulin serum proteins. Based on solid-phase enzyme immunoassay, this antibody cross-reacts with horse IgG, human IgM, swine IgG (6%), cat IgG, chicken IgG, dog IgG, dog IgM, guinea pig IgG, mouse IgM, swine IgM (3%), mouse IgG, rabbit IgG, rat IgG, rat IgM (1%), and it has minimal cross-reactivity with immunoglobulins from other species.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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