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Invitrogen
SKP1 Monoclonal Antibody (1C10F4)
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FIGURE: 1 / 3
SKP1 Antibody (32-3800) in WB
Western blot analysis of SKP-1/p19A and Cullin-1 was performed by loading 10 µg of whole cell lysates from DMSO, MLN4924 or MG132 treated U2OS cells onto a SDS PAGE gel. Proteins were transferred, blocked and endogenous SKP-1 (right panel) and Cullin-1 (left panel) were detected using a SKP-1 mouse monoclonal antibody (Product # 32-3800) or CUL-1 mouse monoclonal antibody (Product # 32-2400) at dilutions of 1:1000.
Product Details
32-3800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1C10F4
Immunogen
Recombinant human SKP1 (p19) protein.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
SKP1 / p19A Antibody (1C10F4), Prod# 32-3800 specifically detects SKP1 in human cells. The antibody may also result in weak detection of 2 unknown bands at ~37kD.
Target Information
This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteasome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cyclin A/CDK2-associated p19; Cyclin-A/CDK2-associated protein p19; MGC34403; OCP-2; OCP-II; OCP-II protein; Organ of Corti protein 2; Organ of Corti protein II; p19A; p19skp1; polypeptide 1-like; RNA polymerase II elongation factor-like protein; RNA polymerase II elongation factor-like protein OCP2; S-phase kinase-associated protein 1; SIII; Transcription elongation factor B polypeptide 1-like
Gene Aliases: EMC19; OCP-II; OCP2; p19A; SKP1; SKP1A; TCEB1L
UniProt ID: (Human) P63208
Entrez Gene ID: (Human) 6500
protein binding
beta-catenin binding
protein domain specific binding
cullin family protein binding
molecular function activator activity
ubiquitin ligase complex scaffold activity
F-box domain binding
protein binding, bridging involved in substrate recognition for ubiquitination
ubiquitin ligase activator activity
protein polyubiquitination
chromatin remodeling
ubiquitin-dependent protein catabolic process
protein monoubiquitination
apoptotic process
protein ubiquitination
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
maintenance of protein location in nucleus
axon development
protein K48-linked ubiquitination
positive regulation of epithelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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