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Invitrogen
NPM1 Monoclonal Antibody (FC-61991)
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FIGURE: 1 / 8
NPM1 Antibody (32-5200) in ICC/IF
Immunofluorescence analysis of Nucleophosmin was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Nucleophosmin (FC-61991) Mouse Monoclonal Antibody (Product # 32-5200) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj µ... View More
Product Details
32-5200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Non-human primate,
Rat,
Virus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
FC-61991
Immunogen
NPM/B23 purified from rat hepatoma.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with the C-terminus of Nucleophosmin (B23). Positive controls used: HeLa, K562, Jurkat, MCF-7, and HL60 cell lysates). In western blots the antibody recognizes a band at ~37 kDa.
Target Information
NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B23 antibody; MGC104254; mNPM antibody; mNPM1 antibody; NO38 antibody; NPM; Nucleolar phosphoprotein B23; nucleolar protein B23.1; Nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; Numatrin; numatrin antibody; testicular tissue protein Li 128
Gene Aliases: B23; B23NP; NO38; NPM; NPM1
UniProt ID: (Human) P06748, (Rat) P13084, (Mouse) Q61937
Entrez Gene ID: (Human) 4869, (Rat) 25498, (Mouse) 18148
transcription coactivator activity
RNA binding
protein kinase inhibitor activity
protein binding
protein kinase binding
Tat protein binding
histone binding
protein homodimerization activity
ribosomal large subunit binding
ribosomal small subunit binding
poly(A) RNA binding
protein heterodimerization activity
NF-kappaB binding
unfolded protein binding
DNA binding
phosphatidylinositol-3,4,5-trisphosphate binding
rRNA binding
enzyme binding
nucleic acid binding
protein N-terminus binding
chaperone
DNA repair
nucleosome assembly
intracellular protein transport
nucleocytoplasmic transport
response to stress
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
centrosome cycle
signal transduction
cell aging
protein localization
positive regulation of cell proliferation
negative regulation of cell proliferation
negative regulation of centrosome duplication
viral process
regulation of endodeoxyribonuclease activity
CENP-A containing nucleosome assembly
ribosome assembly
negative regulation of apoptotic process
negative regulation of protein kinase activity by regulation of protein phosphorylation
positive regulation of translation
positive regulation of transcription, DNA-templated
regulation of centriole replication
positive regulation of NF-kappaB transcription factor activity
protein oligomerization
regulation of endoribonuclease activity
regulation of eIF2 alpha phosphorylation by dsRNA
positive regulation of cell cycle G2/M phase transition
ribosomal large subunit export from nucleus
ribosomal small subunit export from nucleus
rRNA export from nucleus
cell volume homeostasis
regulation of centrosome duplication
positive regulation of centrosome duplication
cell growth
positive regulation of cellular biosynthetic process
protein destabilization
ribosomal large subunit biogenesis
ribosomal small subunit biogenesis
positive regulation of catalytic activity
regulation of DNA damage response, signal transduction by p53 class mediator
regulation of neuron apoptotic process
positive regulation of DNA replication
positive regulation of protein kinase activity
negative regulation of mRNA splicing, via spliceosome
positive regulation of DNA metabolic process
protein homooligomerization
regulation of cell cycle
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