Search
Invitrogen
NPM1 Monoclonal Antibody (FC-61991)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 93
NPM1 Antibody (32-5200) in ICC/IF
Immunofluorescence analysis of Nucleophosmin was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Nucleophosmin (FC-61991) Mouse Monoclonal Antibody (Product # 32-5200) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj µ... View More
.jpg?time=20220826)
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
.jpg?time=20220826?time=20220826 "NPM1 Antibody (32-5200) in ICC/IF")
Product Details
32-5200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine,
Chimpanzee,
Hamster,
Human,
Monkey,
Mouse,
Non-human primate,
Rat,
Virus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
FC-61991
Immunogen
NPM/B23 purified from rat hepatoma.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with the C-terminus of Nucleophosmin (B23). Positive controls used: HeLa, K562, Jurkat, MCF-7, and HL60 cell lysates). In western blots the antibody recognizes a band at ~37 kDa.
Target Information
NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B23 antibody; deletion in NPMc- AML; MGC104254; mNPM antibody; mNPM1 antibody; mutation A in NPMc+ AML; mutation B in NPMc+ AML; mutation C in NPMc+ AML; mutation D in NPMc+ AML; mutation E in NPMc+ AML; mutation F in NPMc+ AML; NO38 antibody; NPM; nucleolar phosphoprotein B23; nucleolar protein B23; nucleolar protein B23.1; nucleolar protein B23.2; nucleolar protein NO38; nucleophosmin (nucleolar phosphoprotein B23 numatrin) isoform NPM1_1; nucleophosmin (nucleolar phosphoprotein B23 numatrin) isoform NPM1_2; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin/nucleoplasmin family, member 1; numatrin; numatrin antibody; testicular tissue protein Li 128; unnamed protein product
Gene Aliases: B23; B23NP; NO38; NPM
UniProt ID: (Human) P06748, (Mouse) Q61937, (Rat) P13084
Entrez Gene ID: (Human) 4869, (Mouse) 18148, (Rat) 25498
core promoter sequence-specific DNA binding
nucleic acid binding
chromatin binding
transcription coactivator activity
RNA binding
protein kinase inhibitor activity
protein binding
rRNA binding
protein kinase binding
Tat protein binding
histone binding
identical protein binding
protein homodimerization activity
ribosomal large subunit binding
ribosomal small subunit binding
NF-kappaB binding
binding, bridging
DNA-binding transcription factor binding
p53 binding
DNA binding
ATP binding
phosphatidylinositol-3,4,5-trisphosphate binding
enzyme binding
protein kinase B binding
ribosomal large subunit export from nucleus
ribosomal small subunit export from nucleus
cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
regulation of cell growth
DNA repair
nucleosome assembly
chromatin remodeling
protein import into nucleus
cell volume homeostasis
nucleocytoplasmic transport
centrosome cycle
I-kappaB kinase/NF-kappaB signaling
protein localization
positive regulation of cell proliferation
negative regulation of cell proliferation
rRNA transcription
posttranscriptional regulation of gene expression
negative regulation of gene expression
negative regulation of cardiac muscle cell apoptotic process
regulation of centrosome duplication
positive regulation of centrosome duplication
negative regulation of centrosome duplication
macrophage differentiation
positive regulation of protein ubiquitination
regulation of protein stability
cellular response to UV
regulation of cell proliferation
ribosomal large subunit biogenesis
ribosomal small subunit biogenesis
negative regulation of apoptotic process
positive regulation of catalytic activity
regulation of DNA damage response, signal transduction by p53 class mediator
regulation of neuron apoptotic process
negative regulation of neuron apoptotic process
negative regulation of protein kinase activity by regulation of protein phosphorylation
positive regulation of translation
positive regulation of DNA replication
positive regulation of protein kinase activity
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of centriole replication
negative regulation of mRNA splicing, via spliceosome
negative regulation of epithelial cell proliferation
protein stabilization
positive regulation of DNA metabolic process
regulation of cell cycle
regulation of eIF2 alpha phosphorylation by dsRNA
cellular senescence
positive regulation of DNA-directed DNA polymerase activity
regulation of mRNA stability involved in cellular response to UV
positive regulation of cell cycle G2/M phase transition
positive regulation of protein localization to nucleolus
positive regulation of cytoplasmic translation
intracellular protein transport
signal transduction
ribosome biogenesis
ribosome assembly
cardiac muscle hypertrophy
cellular response to testosterone stimulus
cellular response to hypoxia
liver regeneration
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support