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Invitrogen
SRSF3 Monoclonal Antibody (7B4 (7B4A12))
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FIGURE: 1 / 30
SRSF3 Antibody (33-4200) in WB
Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), HCT 116 (Lane 2), HT-29 (Lane 3), A549 (Lane 4) and Hep G2 (Lane 5). The blot was probed with Anti-SRP20 Mouse Monoclonal Antibody (Product # 33-4200, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A ~25 kDa band corresponding to SRP20 was observed across the cell lines tested. Known quantity of protein samples were electr... View More
Product Details
33-4200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
7B4 (7B4A12)
Immunogen
A synthetic peptide corresponding to amino acids 84-104 of the human SRp20 protein.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
SR proteins appear to be recruited from nuclear "speckles", in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: pre-mRNA splicing factor SRp20; Pre-mRNA-splicing factor SRP20; Serine/arginine-rich splicing factor 3; Splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich, 20-kD
Gene Aliases: SFRS3; SRP20; SRSF3
UniProt ID: (Human) P84103
Entrez Gene ID: (Human) 6428
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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