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Invitrogen
SOX2 Monoclonal Antibody (Btjce), eFluor™ 570, eBioscience™
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
41-9811-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
Btjce
Conjugate
eFluor™ 570
eFluor™ 570
eFluor™ 570
Excitation/Emission Max
556/569 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, and in particular, Sox2 is essential for regulating genes that control normal mammalian embryogenesis. Sox2 and family member Sox3 are expressed as early as the preimplantation and epiblast stages respectively. Later expression is restricted to the neuroepithelium. Sox2 has been shown to be necessary for maintaining self-renewal and pluripotency of mouse and human embryonic stem (ES) cells (ESC). Oct4 (POU5F1), Klf4, c-myc, and Sox2 were the original four factors used to reprogram differentiated mouse and human cells to induced pluripotent stem cells (iPSC).
Expression of Sox2 is tightly regulated and recent studies have demonstrated that small changes in the levels of Sox2 in ES cells can trigger differentiation into multiple cell types. Sox2 expression is not limited to ES cells, it is also essential for early neurogenesis where its expression becomes restricted to the neural plate, and later to neural stem cells where it functions to suppress neural differentiation. Sox2 in combination with other stem cell markers can be used to characterize stem cell populations. Ectopic expression of Sox2 has been associated with multiple cancer types including colorectal and breast.
Applications Reported: This Btjce antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This Btjce antibody has been tested by immunocytochemistry of fixed and permeabilized F9 cells. Thsi can be used at less than 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.
Excitation: 555 nm; Emission: 570 nm
Bacterial expression of region after aa135 (homology between species but not to other family members)
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); putative; sex determining region Y-box 2; SOX 2; sox-2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY related HMG box 2; SRY-box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; transcription factor SOX2; unnamed protein product
Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; ysb
UniProt ID: (Human) P48431, (Mouse) P48432
Entrez Gene ID: (Human) 6657, (Mouse) 20674
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
miRNA binding
sequence-specific DNA binding
chromatin binding
chromatin DNA binding
nitric-oxide synthase binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
DNA-binding transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
cell fate specification
endodermal cell fate specification
positive regulation of neuroblast proliferation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
brain development
sensory perception of sound
gene expression
regulation of gene expression
stem cell population maintenance
forebrain neuron differentiation
adenohypophysis development
cerebral cortex development
positive regulation of cell-cell adhesion
negative regulation of Wnt signaling pathway
neuron differentiation
male genitalia development
positive regulation of epithelial cell differentiation
forebrain development
olfactory placode formation
response to retinoic acid
somatic stem cell population maintenance
inner ear morphogenesis
positive regulation of MAPK cascade
tongue development
cell fate commitment
negative regulation of cell differentiation
positive regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of neuron differentiation
negative regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
pigment biosynthetic process
lung alveolus development
embryonic organ development
anatomical structure formation involved in morphogenesis
neuron fate commitment
inner ear development
diencephalon morphogenesis
stem cell differentiation
regulation of neurogenesis
detection of mechanical stimulus involved in sensory perception of sound
detection of mechanical stimulus involved in equilibrioception
retina morphogenesis in camera-type eye
lens induction in camera-type eye
epithelial tube branching involved in lung morphogenesis
response to growth factor
cellular response to cadmium ion
negative regulation of canonical Wnt signaling pathway
response to oxygen-glucose deprivation
neuronal stem cell population maintenance
negative regulation of cell cycle G1/S phase transition
regulation of myofibroblast cell apoptotic process
eye development
chromatin organization
response to wounding
glial cell fate commitment
pituitary gland development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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