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Invitrogen
Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 615, eBioscience™
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Product Details
42-4301-80
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
10 µg/mL
Immunohistochemistry (Frozen) (IHC (F))
1-10 µg/mL
Immunocytochemistry (ICC/IF)
0.5 µg/mL
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eBRG1
Conjugate
Excitation/Emission Max
595/614 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This eBRG1 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded and frozen tissue sections and immunocytochemistry.
Applications Tested: This eBRG1 antibody has been tested by immunocytochemistry on fixed and permeabilized cells. Isotype control should be used at the same concentration as experimental antibody. This product has not been validated for flow cytometric analysis.
Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable.
Excitation: 595 nm; Emission: 615 nm.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics. These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants. Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens. Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases. With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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