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Invitrogen
Rat IgG1 kappa Isotype Control (eBRG1), eBioscience™
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FIGURE: 1 / 3
Rat IgG1 kappa Isotype Control (14-4301-82) in IHC (F)
Immunohistochemistry of frozen mouse intestine stained with 2.5 µg/mL Rat IgG1 K Isotype Control Purified (left) or 2.5 µg/mL Anti-Mouse Lyve-1 Purified (right) followed by 5 µg/mL Anti-Rat IgG1 eFluor® 615. Nuclei are stained with DAPI.
Product Details
14-4301-82
Applications
Tested Dilution
Immunohistochemistry (Frozen) (IHC (F))
Assay-Dependent
Immunocytochemistry (ICC/IF)
Assay-Dependent
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eBRG1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This rat IgG1 isotype control has been reported for use in immunohistochemistry, immunocytochemistry, flow cytometric analysis, and immunoglobulin ELISA.
Applications Tested: This rat IgG1 isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and should be used at the same concentration as the experimental antibody.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies that are commonly used in research and diagnostics. These antibodies are produced by rats in response to an antigen and can be purified from serum or hybridoma cell culture supernatants. Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is also used in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens. Gene targeting is a powerful tool used to manipulate the rat genome and generate animal models for studying human diseases. With gene targeting, specific genes can be knocked out, inserted, or modified to investigate their role in disease pathogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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