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Phospho-Vinculin (Tyr100) Polyclonal Antibody
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FIGURE: 1 / 4
Phospho-Vinculin (Tyr100) Antibody (44-1074G) in ICC/IF
Immunofluorescence analysis of Phospho-Vinculin pTyr100 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Vinculin pTyr100 Rabbit Polyclonal Antibody (Product # 44-1074G) at 1:250 dilution in 0.1%... View More
Product Details
44-1074G
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 100. The sequence is conserved in mouse and chicken.
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.3, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 114; epididymis secretory sperm binding protein; meta-vinculin; Metavinculin; MV; RP11-178G16.3; unnamed protein product; vinc; vinc 1; Vinculin
Gene Aliases: CMD1W; CMH15; HEL114; MV; MVCL; VCL; VINC; VINC1
UniProt ID: (Chicken) P12003, (Human) P18206
Entrez Gene ID: (Chicken) 396422, (Human) 7414
dystroglycan binding
structural molecule activity
protein binding
beta-catenin binding
vinculin binding
ubiquitin protein ligase binding
protein homodimerization activity
alpha-catenin binding
cadherin binding
actin filament binding
muscle alpha-actinin binding
alpha-actinin binding
scaffold protein binding
actin binding
binding, bridging
morphogenesis of an epithelium
lamellipodium assembly
regulation of cell migration
adherens junction assembly
protein localization to cell surface
apical junction assembly
axon extension
regulation of focal adhesion assembly
epithelial cell-cell adhesion
regulation of establishment of endothelial barrier
regulation of protein localization to cell-cell adherens junction
cell adhesion
cell-matrix adhesion
negative regulation of cell migration
maintenance of blood-brain barrier
platelet aggregation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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