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          • Primary Antibodies ›
          • FAK Antibodies

          Invitrogen

          Phospho-FAK (Tyr576) Polyclonal Antibody

          Advanced Verification
          12 Published Figures
          24 References
          View all (105) FAK antibodies

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          Cite Phospho-FAK (Tyr576) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          • Published Figures (12)
          • Advanced Verification (1)
          Phospho-FAK (Tyr576) Antibody
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          Phospho-FAK (Tyr576) Antibody
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          Phospho-FAK (Tyr576) Antibody (44-652G)

          Focal Adhesion Kinase (FAK) Protein Schematic-Focal Adhesion Kinase (FAK) is a non-receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion; however, dephosphor... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-FAK (Tyr576) Antibody
          Phospho-FAK (Tyr576) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-FAK (Tyr576) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Phospho-FAK (Tyr576) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Phospho-FAK (Tyr576) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-FAK (Tyr576) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Phospho-FAK (Tyr576) Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          Phospho-FAK (Tyr576) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr576) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr576) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr576) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr576) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr576) Antibody in Western Blot (WB)
          Phospho-FAK (Tyr576) Antibody
          Phospho-FAK (Tyr576) Polyclonal Antibody

          Product Details

          44-652G

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 17 publications 17 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          -
          View 2 publications 2 publications

          Immunoprecipitation (IP)

          -
          View 3 publications 3 publications

          Miscellaneous PubMed (Misc)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Chicken, Human, Mouse, Rat, Xenopus

          Published species

          Human, Mouse, Xenopus, Zebrafish

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 576. The sequence is conserved in mouse, rat, chicken and frog.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533709

          Target Information

          Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: EC 2.7.10.2; FADK 1; FAK-Del33; exon 33 deletion splicing mutant; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase pp125FAK; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; p41/p43FRNK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; tyrosine kinase; unnamed protein product

          View more View less

          Gene Aliases: FADK; FADK 1; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2; XELAEV_18032408mg; XPFAK

          View more View less

          UniProt ID: (Human) Q05397, (Mouse) P34152, (Rat) O35346, (Xenopus) Q91738

          View more View less

          Entrez Gene ID: (Human) 5747, (Chicken) 396416, (Mouse) 14083, (Rat) 25614, (Xenopus) 399286

          View more View less

          Function(s)
          nucleotide binding actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein tyrosine phosphatase activity integrin binding protein binding ATP binding JUN kinase binding kinase activity transferase activity protein kinase binding protein phosphatase binding SH2 domain binding molecular function activator activity protease binding calcium-dependent cysteine-type endopeptidase activity receptor binding identical protein binding signal transducer activity transferase activity, transferring phosphorus-containing groups protein complex binding phosphatidylinositol 3-kinase binding
          Process(es)
          MAPK cascade microtubule cytoskeleton organization angiogenesis blood vessel development vasculogenesis neuron migration regulation of protein phosphorylation positive regulation of protein phosphorylation protein phosphorylation movement of cell or subcellular component cell adhesion signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway JNK cascade multicellular organism development positive regulation of cell proliferation regulation of cell shape regulation of epithelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration phosphorylation peptidyl-tyrosine phosphorylation central nervous system neuron axonogenesis negative regulation of cell-cell adhesion regulation of cell adhesion extracellular matrix organization positive regulation of cell growth positive regulation of cell migration negative regulation of cell migration cellular chloride ion homeostasis regulation of cell adhesion mediated by integrin peptidyl-tyrosine autophosphorylation establishment of nucleus localization regulation of cell proliferation negative regulation of apoptotic process endothelial cell migration innate immune response regulation of osteoblast differentiation positive regulation of cell adhesion positive regulation of protein kinase activity positive regulation of vasodilation negative regulation of organ growth protein autophosphorylation ephrin receptor signaling pathway positive regulation of smooth muscle cell proliferation positive regulation of phagocytosis negative regulation of axonogenesis positive regulation of synaptic transmission regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of synapse assembly positive regulation of glial cell proliferation growth hormone receptor signaling pathway cellular response to transforming growth factor beta stimulus regulation of substrate adhesion-dependent cell spreading positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of anoikis placenta development regulation of cell-matrix adhesion heart morphogenesis axon guidance regulation of endothelial cell migration positive regulation of epithelial cell migration positive regulation of epithelial to mesenchymal transition positive regulation of macrophage chemotaxis positive regulation of fibroblast migration cell migration establishment of cell polarity cell differentiation detection of muscle stretch netrin-activated signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis regulation of GTPase activity regulation of cell differentiation vascular endothelial growth factor receptor signaling pathway cell motility regulation of multicellular organismal process regulation of cytoskeleton organization positive regulation of wound healing vascular endothelial cell response to oscillatory fluid shear stress positive regulation of macrophage proliferation cytoskeleton organization response to mechanical stimulus response to glucose response to organic substance response to organonitrogen compound response to organic cyclic compound response to estradiol response to drug fat cell differentiation response to arsenic-containing substance proteolysis actin filament organization transmembrane receptor protein tyrosine kinase signaling pathway response to pH negative regulation of cell-substrate adhesion pyramidal neuron migration negative regulation of protein autophosphorylation positive regulation of protein binding response to muscle stretch wound healing, spreading of cells positive regulation of focal adhesion assembly angiogenesis involved in wound healing positive regulation of protein tyrosine kinase activity positive regulation of substrate-dependent cell migration, cell attachment to substrate
          It has to be done as per old AB suggested Products section.

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