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Invitrogen

IL-6 Monoclonal Antibody (MQ2-13A5), PerCP-eFluor™ 710, eBioscience™

5 Published Figures
4 References
View all (137) IL-6 antibodies

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Product Details

46-7069-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test
View 4 publications 4 publications
Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MQ2-13A5

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11151511

Product Specific Information

Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: This MQ2-13A5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MQ2-13A5 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; hybridoma growth factor peptide; IFN-beta-2; IL-6; IL6; ILN; interferon 6 precursor; Interferon beta-2; Interleukin; interleukin 6; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6; unnamed protein product

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Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231

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Entrez Gene ID: (Human) 3569

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Function(s)
cytokine activity interleukin-6 receptor binding protein binding growth factor activity identical protein binding
Process(es)
neutrophil apoptotic process germinal center B cell differentiation hepatic immune response neutrophil mediated immunity monocyte chemotaxis positive regulation of immunoglobulin production positive regulation of acute inflammatory response positive regulation of leukocyte chemotaxis acute-phase response inflammatory response immune response humoral immune response JAK-STAT cascade positive regulation of cell proliferation negative regulation of cell proliferation vascular endothelial growth factor production regulation of vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of epithelial to mesenchymal transition negative regulation of lipid storage response to activity cytokine-mediated signaling pathway cell differentiation platelet activation endocrine pancreas development neuron projection development response to peptidoglycan negative regulation of chemokine production positive regulation of chemokine production positive regulation of interleukin-1 beta production positive regulation of interleukin-10 production positive regulation of interleukin-17 production positive regulation of interleukin-21 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production negative regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation maintenance of blood-brain barrier positive regulation of T cell proliferation glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of MAPK cascade negative regulation of fat cell differentiation positive regulation of osteoblast differentiation positive regulation of translation regulation of angiogenesis negative regulation of bone resorption positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade positive regulation of smooth muscle cell proliferation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of neurogenesis regulation of insulin secretion defense response to Gram-negative bacterium defense response to Gram-positive bacterium positive regulation of B cell activation regulation of protein transport positive regulation of lymphocyte activation response to glucocorticoid defense response to virus positive regulation of glial cell proliferation T follicular helper cell differentiation regulation of astrocyte activation neuron cellular homeostasis glucagon secretion regulation of glucagon secretion interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide cellular response to lipopolysaccharide T-helper 17 cell lineage commitment hepatocyte proliferation positive regulation of extracellular matrix disassembly inflammatory response to wounding liver regeneration receptor signaling pathway via STAT cellular response to virus regulation of neuroinflammatory response positive regulation of neuroinflammatory response positive regulation of cytokine production involved in inflammatory response positive regulation of platelet aggregation positive regulation of apoptotic DNA fragmentation positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of microglial cell activation positive regulation of STAT cascade positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of T-helper 2 cell cytokine production negative regulation of primary miRNA processing negative regulation of interleukin-1-mediated signaling pathway positive regulation of type B pancreatic cell apoptotic process
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