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Invitrogen
CD39 Monoclonal Antibody (24DMS1), APC-eFluor™ 780, eBioscience™
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FIGURE: 1 / 1
CD39 Antibody (47-0391-82) in Flow
C57BL/6 mouse splenocytes were stained with Foxp3 Monoclonal Antibody, PE (Product # 12-5773-82) and 1.0 µg of Rat IgG2b kappa Isotype Control, APC-eFluor 780 (Product # 47-4031-82) (left) or 1.0 µg of CD39 Monoclonal Antibody, APC-eFluor 780 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
47-0391-82
Product Specifications
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
24DMS1
Conjugate
Excitation/Emission Max
756/785 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: The 24DMS1 monoclonal antibody reacts with mouse CD39, also known as NTPDaseI. E-NTPDases are enzymes that convert nucleoside tri- and diphosphates (NTDPs) into nucleoside monophosphate (NMP), thereby removing toxic extracellular ATP and ADP. CD39 is the dominant member of this family in the immune system and is involved in suppression of inflammation and control of platelet activation. CD39 can impact expression of CD73, another E-NTPase. Together, these molecules influence inflammation responses. CD39 is expressed on B cells, Langerhans cells and most monocytes. In addition, CD39 is found on a subset of CD4+ T cells that are mostly CD25+FoxP3+ T reg cells. T reg cells from CD39-null mice showed impaired suppressive properties in vitro and in vivo.
Applications Reported: This 24DMS1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 24DMS1 antibody has been pre-diluted and tested by flow cytometric analysis of mouse splenocytes. This may be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser
Target Information
CD39 is an integral membrane protein characterized by two transmembrane domains and forms a homotetramer structure. It is recognized as the most prominent ectoenzyme within the immune system, primarily functioning to remove toxic extracellular ATP by converting it into ADP or AMP. CD39 works in conjunction with CD73 to hydrolyze ATP and is well characterized on Langerhans cells. Originally identified on activated lymphocytes, CD39 is expressed on a variety of immune cells, including peripheral blood B cells, monocytes, and T cell clones. It is also found on a subset of T cells, B cells, and dendritic cells, with weaker expression observed on monocytes and granulocytes. The CD39 cell surface antigen is a 70-100kD molecule, and its expression is induced on T cells and increases on B cells as a late activation antigen. CD39 possesses intrinsic ecto-ATPase activity, playing a crucial role in modulating extracellular nucleotide levels and contributing to immune regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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