CD61 (Integrin beta 3) Monoclonal Antibody (VI-PL2), APC-eFluor™ 780, eBioscience™

Product Specific Information

Description: The VI-PL2 monoclonal antibody reacts with human CD61, also known as integrin beta 3 and GPIIIa. CD61 is a 90-110 kDa member of the beta integrin family expressed by a wide variety of cells, including leukocytes, platelets, endothelial and smooth muscle cells. CD61 binds non-covalently with the alpha integrins CD41 and CD51, to form the alpha IIb beta3 (CD41/CD61) and alpha v beta3 (CD51/CD61) complexes. These alpha beta heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. The CD41/CD61 integrin has been shown to be involved in platelet aggregation, and binds to fibrinogen, von Willebrand Factor (vWF) and fibronectin. The CD51/CD61 integrin binds to matrix proteins including vitronectin, fibronectin, vWF and fibrinogen, and has been shown to have a strong role in modulating the migration and survival of angiogenic endothelial cells.

This VI-PL2 antibody has also been shown to crossreact with canine and several non-human primate species, including baboon, rhesus, and cynomologus monkey.

Applications Reported: This VI-PL2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This VI-PL2 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor® emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Target Information

CD61, also known as GPIIIa or ITGB3, is a 105 kDa glycoprotein expressed on activated T cells, granulocytes, megakaryocytes, platelets, and their precursors. It plays a crucial role in platelet aggregation and functions as a receptor for fibrinogen, fibronectin, von Willebrand factor, vitronectin, and thrombospondin. CD61 forms heterodimeric complexes by associating non-covalently with integrin alpha subunits: alphaV (CD51) to create the Vitronectin Receptor and alphaIIb (CD41) to form gpIIb/IIIa. These complexes are responsible for adhesion to extracellular matrix components, facilitating cell adhesion and cell-surface mediated signaling. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, and osteoclasts in association with CD51. The protein product of CD61 is composed of an alpha chain and a beta chain, which can combine with multiple partners to form different integrins. Its involvement in cell adhesion and signaling underscores its importance in normal physiological processes. Dysfunction of CD61 is associated with diseases such as Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder, highlighting its critical role in hemostasis and platelet function.