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IL-6 Monoclonal Antibody (MP5-20F3), eFluor™ 450, eBioscience™
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FIGURE: 1 / 27
IL-6 Antibody (48-7061-82) in Flow
Mouse thioglycolate-elicited peritoneal exudate cells were either unstimulated (left) or stimulated with LPS in the presence of Brefeldin A (Product # 00-4506-51) overnight (right). The cells were surface stained with Anti-Mouse F4/80 Antigen eFluor® 660 (Product # 50-4801-82) and intracellularly stained with 0.25 µg of Anti-Mouse IL-6 eFluor® 450 using the Intracellular Fixation & Perbeabilization Buffer Set (Product # 88-8824-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), ... View More
Product Details
48-7061-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MP5-20F3
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.
Applications Reported: This MP5-20F3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This MP5-20F3 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse thioglycolate-elicited peritoneal exudate cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6
Gene Aliases: Il-6; Il6
UniProt ID: (Mouse) P08505
Entrez Gene ID: (Mouse) 16193
neutrophil apoptotic process
myeloid cell homeostasis
hepatic immune response
positive regulation of acute inflammatory response
negative regulation of protein kinase activity
acute-phase response
inflammatory response
immune response
positive regulation of cell proliferation
negative regulation of cell proliferation
regulation of cell shape
response to wounding
regulation of vascular endothelial growth factor production
positive regulation of gene expression
cell growth
cytokine-mediated signaling pathway
endocrine pancreas development
neuron projection development
positive regulation of chemokine production
positive regulation of interleukin-6 production
negative regulation of collagen biosynthetic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of protein import into nucleus, translocation
positive regulation of T cell proliferation
T cell activation
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat3 protein
glucose homeostasis
defense response to protozoan
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAPK cascade
negative regulation of chemokine biosynthetic process
regulation of circadian sleep/wake cycle, non-REM sleep
positive regulation of nitric oxide biosynthetic process
cell redox homeostasis
positive regulation of T-helper 2 cell differentiation
positive regulation of neuron differentiation
negative regulation of gluconeogenesis
positive regulation of translation
positive regulation of DNA replication
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
muscle cell cellular homeostasis
negative regulation of hormone secretion
negative regulation of muscle organ development
positive regulation of smooth muscle cell proliferation
positive regulation of epithelial cell proliferation
negative regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of B cell activation
positive regulation of immunoglobulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
response to glucocorticoid
defense response to virus
positive regulation of protein kinase B signaling
positive regulation of transmission of nerve impulse
branching involved in salivary gland morphogenesis
epithelial cell proliferation involved in salivary gland morphogenesis
glucagon secretion
interleukin-6-mediated signaling pathway
cellular response to hydrogen peroxide
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to interleukin-1
cellular response to tumor necrosis factor
T-helper 17 cell lineage commitment
negative regulation of neuron death
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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