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IL-9 Monoclonal Antibody (MH9D1), eFluor™ 450, eBioscience™
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FIGURE: 1 / 2
IL-9 Antibody (48-7098-42) in Flow
Th9-polarized normal human peripheral blood cells were stimulated with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) and then intracellularly stained with Anti-Human CD4 FITC (Product # 11-0049-42) and Mouse IgG2a K Isotype Control eFluor® 450 (Product # 48-4724-82) (left) or Anti-Human IL-9 eFluor® 450 (right), using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Viable cells in the lymphocyte gate, as determined by Fixable Viability Dye eFluor® 660 ... View More
Product Details
48-7098-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MH9D1
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody MH9D1 recognizes interleukin-9 (IL-9), a proinflammatory cytokine historically believed to be involved in type 2 immune responses. Recent evidence suggests IL-9 may instead be secreted by a distinct T-helper lineage called Th9. These cells can be derived from Th2 cells with TGF beta or differentiated directly from naive CD4+ T cells with TGF beta and IL-4.
IL-9 is a member of the common cytokine receptor gamma chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders. Staining with the MH9D1 antibody is not blocked by the MH9A4 antibody, suggesting that the two antibodies bind to unique epitopes.
Applications Reported: This MH9D1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This MH9D1 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Th9 polarized human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This antibody can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytokine P40; homolog of mouse T cell and mast cell growth factor 40; homolog of mouse T cell and mast cell growth factor P40; IL-9; ILN; Interleukin; Interleukin-9; Interleukin9; p40 cytokine; P40 protein precursor; p40 T-cell and mast cell growth factor; T-cell growth factor P40
Gene Aliases: HP40; IL-9; IL9; P40
UniProt ID: (Human) P15248
Entrez Gene ID: (Human) 3578
inflammatory response
immune response
positive regulation of cell proliferation
immunoglobulin mediated immune response
B cell differentiation
positive regulation of cell growth
positive regulation of interleukin-5 production
interleukin-9-mediated signaling pathway
B cell proliferation
regulation of JAK-STAT cascade
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