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IL-13 Monoclonal Antibody (85BRD), eFluor™ 450, eBioscience™
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FIGURE: 1 / 1
IL-13 Antibody (48-7136-42) in Flow
Normal human peripheral blood cells were stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) and then stained with CD4 Monoclonal Antibody, FITC (Product # 11-0049-42). Cells were then stained intracellularly using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with Mouse IgG2b kappa Isotype Control, eFluor 450 (Product # 48-4732-82) (left) or IL-13 Monoclonal Antibody, eFluor 450 (right). Cells in the lymphocyte gate were used f... View More
Product Details
48-7136-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
85BRD
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 85BRD monoclonal antibody reacts with human interleukin-13 (IL-13). IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression.
Applications Reported: This 85BRD antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 85BRD antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins." This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30; NC30 antibody; P600 antibody
Gene Aliases: IL-13; IL13; NC30; P600
UniProt ID: (Human) P35225
Entrez Gene ID: (Human) 3596
microglial cell activation
positive regulation of immunoglobulin production
inflammatory response
immune response
transforming growth factor beta receptor signaling pathway
response to mechanical stimulus
response to nematode
regulation of proton transport
response to selenium ion
positive regulation of gene expression
positive regulation of B cell proliferation
response to lipopolysaccharide
positive regulation of interleukin-10 production
transforming growth factor beta1 production
response to nicotine
interleukin-13-mediated signaling pathway
macrophage activation
positive regulation of macrophage activation
positive regulation of ion transport
positive regulation of mast cell degranulation
response to ethanol
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
positive regulation of protein secretion
negative regulation of inflammatory response
positive regulation of release of sequestered calcium ion into cytosol
cellular response to mechanical stimulus
cellular response to cytokine stimulus
negative regulation of transforming growth factor beta production
positive regulation of cold-induced thermogenesis
negative regulation of lung ciliated cell differentiation
positive regulation of lung goblet cell differentiation
negative regulation of complement-dependent cytotoxicity
positive regulation of pancreatic stellate cell proliferation
negative regulation of endothelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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