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          • Primary Antibodies ›
          • HTR2A/HTR2B/HTR2C Antibodies

          Bioss

          5-HTR2 Polyclonal Antibody

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          Cite 5-HTR2 Polyclonal Antibody

          • Antibody Testing Data (3)
          5-HTR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          5-HTR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          5-HTR2 Antibody (BS-1056R) in IHC (P)

          Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with 5-HT2A Polyclonal Antibody, Unconjugated (bs-1056R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          5-HTR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          5-HTR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          5-HTR2 Antibody in Western Blot (WB)

          Product Details

          BS-1056R

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:200
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:400
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:100-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          Flow Cytometry (Flow)

          1:20-1:100
          -

          ELISA (ELISA)

          1:500-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KLH conjugated synthetic peptide derived from human 5-HTR2A.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

          Contains

          0.02% ProClin 300

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca(2+) ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: 5-HT 2B receptor; 5-HT-1C; 5-HT-2; 5-HT-2A; 5-HT-2B; 5-HT-2C; 5-HT-2F; 5-HT1C; 5-HT2 receptor; 5-HT2A receptor; 5-HT2B; 5-HT2C receptor; 5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled; 5-hydroxytryptamine (serotonin) receptor 2B, G protein-coupled; 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled; 5-hydroxytryptamine 2 receptor; 5-hydroxytryptamine 2B receptor; 5-hydroxytryptamine receptor 1C; 5-hydroxytryptamine receptor 2A; 5-hydroxytryptamine receptor 2B; 5-hydroxytryptamine receptor 2B variant b; 5-hydroxytryptamine receptor 2C; NP75 protein; serotonin 1c receptor; serotonin 5-HT-1C receptor; serotonin 5-HT-2A receptor; serotonin 5-HT-2C receptor; serotonin 5HT-2 receptor; Serotonin receptor 2A; Serotonin receptor 2B; Serotonin receptor 2C; Stomach fundus serotonin receptor

          View more View less

          Gene Aliases: 5-HT(2B); 5-HT-2B; 5-HT1C; 5-HT2A; 5-HT2B; 5-HT2C; 5-HT2cR; 5-HTR2C; 5HT-1C; 5Ht-2; 5HT1c; 5HTR2C; AJ012488; AV377389; E030013E04; Htr-2; HTR1C; HTR2; HTR2A; HTR2B; HTR2C; SR1; Srl

          View more View less

          UniProt ID: (Human) P28223, (Human) P41595, (Human) P28335, (Mouse) P35363, (Rat) P14842, (Mouse) Q02152, (Rat) P30994, (Rat) P08909, (Mouse) P34968

          View more View less

          Entrez Gene ID: (Human) 3356, (Human) 3357, (Human) 3358, (Mouse) 15558, (Rat) 29595, (Mouse) 15559, (Rat) 29581, (Rat) 25187, (Mouse) 15560

          View more View less

          Function(s)
          virus receptor activity G-protein alpha-subunit binding G-protein coupled serotonin receptor activity drug binding protein complex binding serotonin binding 1-(4-iodo-2,5-dimethoxyphenyl)propan-2-amine binding GTPase activator activity Gq/11-coupled serotonin receptor activity signal transducer activity G-protein coupled receptor activity protein binding G-protein coupled receptor
          Process(es)
          temperature homeostasis cellular calcium ion homeostasis activation of phospholipase C activity phospholipase C-activating serotonin receptor signaling pathway serotonin receptor signaling pathway chemical synaptic transmission aging memory cell death positive regulation of cell proliferation positive regulation of phosphatidylinositol biosynthetic process regulation of dopamine secretion phosphatidylinositol 3-kinase signaling artery smooth muscle contraction urinary bladder smooth muscle contraction sleep response to drug negative regulation of potassium ion transport positive regulation of MAP kinase activity protein localization to cytoskeleton positive regulation of fat cell differentiation positive regulation of glycolytic process positive regulation of vasoconstriction viral entry into host cell regulation of hormone secretion behavioral response to cocaine positive regulation of peptidyl-tyrosine phosphorylation regulation of behavior detection of temperature stimulus involved in sensory perception of pain detection of mechanical stimulus involved in sensory perception of pain release of sequestered calcium ion into cytosol negative regulation of synaptic transmission, glutamatergic positive regulation of ERK1 and ERK2 cascade neural crest cell migration positive regulation of cytokine production positive regulation of endothelial cell proliferation G-protein coupled receptor internalization heart morphogenesis cardiac muscle hypertrophy cGMP biosynthetic process G-protein coupled receptor signaling pathway protein kinase C-activating G-protein coupled receptor signaling pathway behavior negative regulation of autophagy neural crest cell differentiation intestine smooth muscle contraction phosphorylation calcium-mediated signaling hepatic stellate cell activation vasoconstriction negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of GTPase activity inositol phosphate metabolic process embryonic morphogenesis positive regulation of cytokine secretion positive regulation of nitric-oxide synthase activity positive regulation of cell division negative regulation of cell death ERK1 and ERK2 cascade protein kinase C signaling cellular response to amine stimulus cellular response to temperature stimulus cellular response to serotonin behavioral fear response phospholipase C-activating G-protein coupled receptor signaling pathway locomotory behavior feeding behavior regulation of neurological system process regulation of appetite regulation of corticotropin-releasing hormone secretion positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway smooth muscle contraction signal transduction positive regulation of cytosolic calcium ion concentration sensory perception of pain positive regulation of kinase activity heart development ion transmembrane transport G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger positive regulation of gamma-aminobutyric acid secretion positive regulation of acetylcholine secretion, neurotransmission organ regeneration phospholipase D-activating G-protein coupled receptor signaling pathway behavioral response to nicotine negative regulation of locomotion negative regulation of dopamine metabolic process inositol phosphate-mediated signaling regulation of sensory perception of pain
          It has to be done as per old AB suggested Products section.
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