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Invitrogen
5-Hydroxytryptophan Polyclonal Antibody
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FIGURE: 1 / 2
5-Hydroxytryptophan Antibody (PA5-119514) in ELISA
ELISA of 5-Hydroxytryptophan in 100 µL (coated at a concentration of 10 µg/mL) of 5-Hydroxytryptophan carrier protein conjugate. Detection range is 630 ng/mL down to 2.3 ng/mL (coated ligand detection range). Sample was incubated with polyclonal 5-Hydroxytryptophan antibody (Product # PA5-119514), followed by Rabbit IgG (HRP) with a dilution of 1:2000.
Product Details
PA5-119514
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
5-Hydroxytryptophan.
Conjugate
Form
Liquid
Concentration
0.63 mg/mL
Amount
63 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target Information
5-Hydroxytryptophan (5-HTP) is an intermediate metabolite in the biochemical synthesis of serotonin from the essential amino acid tryptophan. The conversion of tryptophan to 5-HTP involves the enzyme tryptophan hydroxylase (TPH), which exists in two isoforms: TPH1 and TPH2. TPH1 is mainly expressed in the peripheral tissues, while TPH2 is predominantly found in the central nervous system. Once synthesized, 5-HTP is further decarboxylated by aromatic L-amino acid decarboxylase (AADC) to produce serotonin (5-hydroxytryptamine, 5-HT). This biogenic amine plays a crucial role in regulating mood, cognition, and various physiological processes. Serotonin can also be acetylated and methylated to produce melatonin, a hormone involved in regulating sleep-wake cycles. Given its central role in serotonin biosynthesis, 5-HTP is often used in the treatment of conditions like depression, anxiety, and insomnia. Recent biotechnological advances have focused on optimizing the production of 5-HTP through engineered enzyme systems to meet increasing demand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-HTP; oxitriptan
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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