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Invitrogen
CD199 (CCR9) Monoclonal Antibody (BBC3M4), eFluor™ 660, eBioscience™
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FIGURE: 1 / 1
CD199 (CCR9) Antibody (50-1999-42) in Flow
CD4+ T cells isolated from normal human perpheral blood cells were stimulated for 3 days with all-trans retinoic acid, Anti-Human CD3, and Anti-Human CD28 Functional Grade Purifieds (Product # 16-0037-81 and Product # 16-0289-81). The cells were cultured for 2 additional days with all-trans retinoic acid and Human IL-2 Recombinant Protein (Product # 14-8029-81). These cells were surface stained with Anti-Human/Mouse Integrin beta 7 FITC (Product # 11-5867-42) and Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or Ant... View More
Product Details
50-1999-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
BBC3M4
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This BBC3M4 monoclonal antibody reacts with human CD199 (CCR9), a 7 transmembrane spanning G-protein-coupled receptor. CCR9 expression is regulated during thymic development and CCR9 deficiency results in mild impairment in thymocyte development. In the periphery, CCR9 and alpha 4 beta 7 integrin are upregulated in response to dendritic cell-derived retinoic acid, conferring preferential trafficking to gut-associated tissues. CCR9 is the receptor for CCL25 (TECK).
Applications Reported: This BBC3M4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This BBC3M4 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C chemokine receptor type 9; C-C CKR-9; CCR9A; CCR9B; CDw199; CDw199 antigen; chemokine (C-C motif) receptor 9; G protein-coupled receptor; G protein-coupled receptor 28; G-protein coupled receptor 28; GPR-9-6; unnamed protein product
Gene Aliases: CC-CKR-9; CCR9; CDw199; GPR-9-6; GPR28
UniProt ID: (Human) P51686
Entrez Gene ID: (Human) 10803
CD8-positive, gamma-delta intraepithelial T cell differentiation
chemotaxis
immune response
cellular defense response
signal transduction
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
calcium-mediated signaling
cell chemotaxis
chemokine-mediated signaling pathway
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